FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005108417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610278
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT41290.25641535188333936No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39110.24287731683597494No Hit
ATGTATTACTGTGCCCTCCAAAGATATTGTATTCGTCACGACTGTTCGGA26050.1617733087081858No Hit
GTATTACTGTGCCCTCCAAAGATATTGTATTCGTCACGACTGTTCGGACT24480.1520234394309554No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA23550.14624803915845588No Hit
CTTATAGTCCGTCCTTCGAAGGCCAGGTCACCTTCTCAGTCGACACGTCC21790.135318249395446No Hit
TCCATAAACACCGCCTACTTACAGTTCAGTAGGCTGAAGTCCTCGGACAC21450.13320681273668275No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20180.12531997580541993No Hit
TCCATACCCTTTCCCGTCGGGCCTCGGGAGTCCCTGACAGATTTAATGGC19950.12389165100684478No Hit
GTAATACATGGCGGTGTCCGAGGACTTCAGCCTACTGAACTGTAAGTAGG19800.12296013483386098No Hit
GATCAGGACTCCTCAGTTCACCTCCTCACCATGAGGCTCCCTGCTCAGCT19600.1217181132698826No Hit
CTATTGGATCGCCTGGGTACGCCAGACGCCCGAGAAAGGCCTGGAGTGGA19490.12103500140969448No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18630.11569430868458737No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18380.1141417817296144No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT17850.11085042458507165No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17400.10805587606612026No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17150.10650334911114726No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT17080.10606864156375484No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA17070.1060065404855559No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17010.1056339340163624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16660.10346039627940021No Hit
GTCTAGAGCAGAAGTGAAAAAGCCCGGAGACTCTCTGAAGATCTCCTGTA16300.10122475746423909No Hit
CCCCTGGAGAGCCGGCCTCCATTTCCTGCAGCTCTAGCCAGAGCCTCTTG16300.10122475746423909No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)