FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775241
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACACAGCCGTGTCCTCACGTCTCAGAGTATTCATTTCAAGATATA81210.45745901542382134No Hit
CTATTATGGCGGTGCTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCC72610.4090148886827197No Hit
GATTTATAGTACGAGCAACAAACACTCCTGGACCCCTGCCCGCTTCTCAG72390.40777562032422643No Hit
CTATTACTACTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCG69560.3918341228036081No Hit
CTCCTGGACCCCTGCCCGCTTCTCAGGCTCCCTCCGTGGGGGCAAAGCCG53590.30187450605298094No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT51020.287397598410582No Hit
GTAGTAATAGTGCGTTGAGACTCTTGAAGCTTTCACACAATAATACACAG47460.26734398315496316No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44530.25083918183502973No Hit
CAATAATACACAGCCGTGTCCTCACGTCTCAGAGTATTCATTTCAAGATA44380.249994226136057No Hit
GTGTATTATTGTGTGAAAGCTTCAAGAGTCTCAACGCACTATTACTACTA43920.2474030286592074No Hit
CCATAATAGAGTAGGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCACACC43820.24683972485989225No Hit
GCCTTGGGCTGACCGAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCCA43820.24683972485989225No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGCCAC39330.22154738427064266No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAATATGTGTC38550.21715361463598465No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA37650.21208388044214843No Hit
GTATTATTGTGTGAAAGCTTCAAGAGTCTCAACGCACTATTACTACTACG37530.21140791588297025No Hit
GTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTACTGCCTACTCTA37430.21084461208365513No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT36480.20549322599016134No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA35920.20233872471399658No Hit
CCGTAGTAGTAATAGTGCGTTGAGACTCTTGAAGCTTTCACACAATAATA35700.20109945635550328No Hit
CTATAAATCAGTGTCCTGGGTGCTTGTCCAGGCTTCTGCTGGAACCAGTT35630.2007051436959827No Hit
TACACATACAACGCAGAGTCTGTGAAGGGCAGATTCAGCATCTCCAGAGA35460.19974752723714695No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34890.1965366955810507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34740.19569173988207797No Hit
GACTATTACTGCCTACTCTATTATGGCGGTGCTTGGGTGTTCGGCGGAGG32280.18183446641892564No Hit
CCGTATATCTTGAAATGAATACTCTGAGACGTGAGGACACGGCTGTGTAT32200.18138382337947354No Hit
CTTTAAAAGATGTCCAGTGTGAGGTGCAATTGGTGGAGTCTGGGGGAGGC31550.17772234868392517No Hit
CCTGACACTGTGAGGGCGGCTTTGCCCCCACGGAGGGAGCCTGAGAAGCG31330.17648308032543186No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA31140.17541280310673313No Hit
CTACTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTACT31000.17462417778769193No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30280.17056839043262295No Hit
ATACACAGCCGTGTCCTCACGTCTCAGAGTATTCATTTCAAGATATACGG30180.17000508663330782No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACACTGTGAGGGCGG29790.1678082018159788No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG29400.16561131699864975No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG29080.16380874484084135No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA29070.16375241446090982No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGCCACTCTCACCTGTGCTTCCA28720.16178085116330684No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28260.1591896536864572No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27950.1574434119085803No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC26360.14848688149946965No Hit
TAGTAATAGTGCGTTGAGACTCTTGAAGCTTTCACACAATAATACACAGC26250.14786724732022302No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA25750.14505072832364732No Hit
GTAATGGGGTTTACACATACAACGCAGAGTCTGTGAAGGGCAGATTCAGC25740.1449943979437158No Hit
GTCCAGGAGTGTTTGTTGCTCGTACTATAAATCAGTGTCCTGGGTGCTTG25640.1444310941444007No Hit
GTTCGTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAA25280.1424032004668662No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG25200.14195255742741408No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA25110.14144558400803045No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24550.1382910827318657No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24240.13654484095398878No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23030.12972886498227565No Hit
CTCCTGTTCAGCCTCTGGATTCACCTTCAGTTCGTATGCTATGCACTGGG22930.1291655611829605No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC22260.1253914257275491No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG21980.12381417508946672No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21900.12336353205001463No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC21630.12184261179186376No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT21550.12139196875241164No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT21470.12094132571295953No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGTAGACGGTGACCGTGGTCCCTTGG21460.12088499533302803No Hit
ACTCTCACCTGTGCTTCCACAACTGGATCAGTCACCAGTGCTTTCTTTCC20830.11733618139734268No Hit
CTCCAGAGACAATGCCAAGAACACCGTATATCTTGAAATGAATACTCTGA20740.11682920797795905No Hit
GAATATGTGTCAGCTATTAGCAGTAATGGGGTTTACACATACAACGCAGA20400.11491397506028758No Hit
GATATACGGTGTTCTTGGCATTGTCTCTGGAGATGCTGAATCTGCCCTTC20250.11406901936131489No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20200.11378736746165732No Hit
CTATTACTGCCTACTCTATTATGGCGGTGCTTGGGTGTTCGGCGGAGGGA19810.1115904826443283No Hit
GTCTGGGGGAGGCTTGGTCCAGCCTGGAGGGTCCCTGAGACTCTCCTGTT19430.1094499282069308No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19310.10877396364775262No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT19280.10860497250795807No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG19250.10843598136816353No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19240.10837965098823202No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC19200.10815432946850596No Hit
GAGTAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCACACCGTAGTA19010.10708405224980722No Hit
TCCTCAGGCTGCACACCTGACACTGTGAGGGCGGCTTTGCCCCCACGGAG18570.10460551553282062No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18360.10342257755425882No Hit
GCTGTGTATTATTGTGTGAAAGCTTCAAGAGTCTCAACGCACTATTACTA18270.10291560413487522No Hit
CCCTGAGACTCTCCTGTTCAGCCTCTGGATTCACCTTCAGTTCGTATGCT18240.10274661299508067No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG18240.10274661299508067No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18100.10195798767603947No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18000.10139468387672436No Hit
GTATGTGTAAACCCCATTACTGCTAATAGCTGACACATATTCCAGTCCCT17870.10066238893761466No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA17860.10060605855768316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)