FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005108507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764274
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCATC179570.6496099880113188No Hit
GGCTATATATATTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCT154950.5605450110951374No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCAGTGATGGCCAGGAAGG148730.538043623750757No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGG144270.5219091884523748No Hit
GTATATTACTGTGCGAAGCAGGGAGGCTGGGAGGGTGGTTACTATGGTTT86960.31458531245455407No Hit
GTAATATACGGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGGAGTTTCA84760.3066266223970561No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA76160.27551537944501886No Hit
GTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCAGGGACCC73740.2667608203817711No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT71450.25847654754919375No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA70590.25536542325399003No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC65750.2378563051274946No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT64140.23203199103996203No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA63090.22823352533070168No Hit
TATATAGCCTGTTTCGATGATGGATCTGCTTCCGGAACAAGAGATGGCGA60710.2196236697230448No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT58480.21155645207385376No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG55230.19979929630709548No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA54180.19600083059783507No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53080.19202148556908613No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT52790.19097238551605233No Hit
GTGTTGACTCAGCCACCCTCAGCGTCAGGGACCCCCGGGCAGAGGGTCGC51770.1872824473984851No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC50500.18268811268347493No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC50470.1825795850917818No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG49970.18077079189689588No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA49650.17961316425216892No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATT48970.1771532055071241No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA47640.17234181560872763No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGGCG46380.1677836567576152No Hit
GTCCAAGACTGCCACCTCAGCCTTCCTGGCCATCACTGGGCTCCGGTCCG46310.1675304257103312No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC46070.16666220497678597No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGACTCAGG45880.1659748635627293No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC45840.16583016010713844No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA45400.16423842209563885No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG44990.16275521167583243No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT42240.15280684910396003No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG41890.1515406938675399No Hit
AATATATATAGCCTGTTTCGATGATGGATCTGCTTCCGGAACAAGAGATG41830.1513236386841536No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41830.1513236386841536No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA40490.14647607292185943No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39620.14332877276275796No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT39380.14246055202921273No Hit
CCTCACTCACTGGGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCAC38960.14094116574550858No Hit
GATAACCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGAGTCCAAGAC38750.14018147260365652No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT38620.13971118637298618No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC38250.13837267940877063No Hit
CCCAAAGACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCA38160.13804709663369116No Hit
CATCTACACGGATAACCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG38130.137938569041998No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA37820.13681711726116877No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA37260.13479126888289655No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGGCGGAG36780.1330548274158061No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC36750.13294629982411296No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT36560.1322589584100563No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC36340.13146308940430654No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT36270.1312098583570225No Hit
ATATATAGCCTGTTTCGATGATGGATCTGCTTCCGGAACAAGAGATGGCG35930.12997987898450009No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC35910.12990752725670465No Hit
GCCGTATATTACTGTGCGAAGCAGGGAGGCTGGGAGGGTGGTTACTATGG35590.1287498996119777No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA35320.1277731512867393No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG35150.1271581616004781No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT34670.12542172013338765No Hit
TCCTCACCCTCCTCACTCACTGGGCAGGGTCCTGGGCCCAGCCTGTGTTG34430.12455349939984241No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG33970.1228894096605474No Hit
GTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC33880.12256382688546795No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA33800.1222744199742862No Hit
ATATTACTGTGCGAAGCAGGGAGGCTGGGAGGGTGGTTACTATGGTTTTG33640.12169560615192271No Hit
ATATACGGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGGAGTTTCATCG33380.12075503369058205No Hit
GCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGGCGGAGGGACCAAGCT33200.12010386814042312No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT32080.11605217138387874No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT31560.11417102646119741No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG30630.11080667111870963No Hit
GTCTTGGACTCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGGTTATC30550.1105172642075279No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29900.10816583305417625No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG29630.10718908472893787No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC29380.10628468813149493No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAG29330.10610380881200633No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC28960.10476530184779077No Hit
ATATATTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCATCTA28940.10469295011999533No Hit
CTATATATATTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCA28500.10310121210849577No Hit
TATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTC28470.1029926845168026No Hit
GTGCAGCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGGCGGAGGGACC28460.10295650865290487No Hit
CAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAGTGATGGCCAGGAA28310.10241387069443912No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCTCCATCTCCAGAGA27910.10096683613853041No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG27880.10085830854683726No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG27780.10049654990786007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)