FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005108508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2764274
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCATC184550.6676255682323822No Hit
GGCTATATATATTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCT149900.54227619982679No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGG143870.5204621538964661No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCAGTGATGGCCAGGAAGG137910.4989013390134263No Hit
GTAATATACGGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGGAGTTTCA81210.29378419071336637No Hit
TATATAGCCTGTTTCGATGATGGATCTGCTTCCGGAACAAGAGATGGCGA78620.2844146419638574No Hit
GTATATTACTGTGCGAAGCAGGGAGGCTGGGAGGGTGGTTACTATGGTTT78170.2827867280884601No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA70830.25623364398753523No Hit
GTCCTGGGCCCAGCCTGTGTTGACTCAGCCACCCTCAGCGTCAGGGACCC68000.2459958745044811No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA67890.2455979400016062No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC67680.2448382468597541No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT65990.23872452586103984No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT65650.2374945464885174No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT65520.2370242602578471No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57900.20945825196778614No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC55450.20059516531284524No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG54600.19752021688153923No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA53170.1923470683441656No Hit
AATATATATAGCCTGTTTCGATGATGGATCTGCTTCCGGAACAAGAGATG51030.18460543347005398No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG50980.18442455415056538No Hit
GTGTTGACTCAGCCACCCTCAGCGTCAGGGACCCCCGGGCAGAGGGTCGC50030.1809878470802822No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT49840.18030050566622557No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA48780.17646586409306747No Hit
ATATATAGCCTGTTTCGATGATGGATCTGCTTCCGGAACAAGAGATGGCG48460.1753082364483405No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA48130.17411443293971582No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA47270.1710033086445121No Hit
GTCCAAGACTGCCACCTCAGCCTTCCTGGCCATCACTGGGCTCCGGTCCG47150.17056919827773948No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC46810.16933921890521705No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGGCG46800.16930304304131935No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA46400.16785600848541063No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG46160.1669877877518654No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGACTCAGG45990.1663727980656042No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44830.16217639785346896No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC44830.16217639785346896No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA44460.16083789088925338No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA44320.16033142879468532No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG41320.14947866962537No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA40750.14741664538320007No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT40540.146656952241348No Hit
GATAACCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGAGTCCAAGAC40070.14495668663815525No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA38680.13992824155637248No Hit
CATCTACACGGATAACCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG38210.13822797595317973No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGGCGGAG37850.1369256448528619No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC37410.13533390684136234No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT37260.13479126888289655No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATT37090.13417627919663536No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA36660.1326207170490335No Hit
CCCAAAGACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCA36120.13066722039855672No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC35760.1293648892982389No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35420.12813490992571647No Hit
GTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCC35090.1269411064170918No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA35060.12683257882539864No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC34590.1251323132222059No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC34440.12458967526374014No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT34340.12422791662476296No Hit
GTCTTGGACTCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGGTTATC34140.1235043993468086No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34100.12335969589121773No Hit
GCATGGGATGACAGCCTGAGTCTTTGGGTGTTCGGCGGAGGGACCAAGCT33730.12202118892700217No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG33620.12162325442412728No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT33580.12147855096853641No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33140.11988681295703682No Hit
GCCGTATATTACTGTGCGAAGCAGGGAGGCTGGGAGGGTGGTTACTATGG32990.11934417499857106No Hit
CCTCACTCACTGGGCAGGGTCCTGGGCCCAGCCTGTGTTGACTCAGCCAC32840.11880153704010529No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA32040.11590746792828786No Hit
ATATACGGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGGAGTTTCATCG31960.11561806101710612No Hit
ATATTACTGTGCGAAGCAGGGAGGCTGGGAGGGTGGTTACTATGGTTTTG31900.11540100583371982No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC31870.11529247824202667No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT31180.11279634363308413No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG30430.11008315384075529No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG30330.10972139520177811No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG29870.1080573054624831No Hit
CTATATATATTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCA29790.10776789855130135No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG29580.10700820540944928No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT29290.10595910535641545No Hit
CTCCTCATCTACACGGATAACCAGCGGCCCTCAGGGGTCCCTGACCGATT29200.105633522581336No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC28840.10433119148101817No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAG28620.10353532247526837No Hit
ATATATTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACTCCTCATCTA28580.1033906190196775No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT28410.1027756293334163No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC28150.10183505687207564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)