FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031115
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATA127010.6253215598328997No Hit
ATCTATTACTGTGCGAGAGATGAAGGTCGCCACGATGACTTAATTACTTA113770.5601356890181008No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT79810.39293688442062613No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC63130.31081450336391586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60470.297718248351275No Hit
CTATTACTGTCAGCAGTACGCTAGGTCGCCGGTCACTTTCGGCGGAGGGA42320.20835846320863172No Hit
GTTCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGG39930.1965915273138153No Hit
GTCTATTACTGTCAGCAGTACGCTAGGTCGCCGGTCACTTTCGGCGGAGG38290.18851714452406682No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37370.18398761271518355No Hit
GTCTCCAGGCTCCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCT35490.1747316129318133No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGACTCTGGGGGAGGCG34580.17025131516433092No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32730.16114301750516344No Hit
GTTACGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC32330.15917365584912718No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32110.15809050693830728No Hit
TCAGTAGACTGGAGCCTGAAGATTTTGCAGTCTATTACTGTCAGCAGTAC31670.15592420911666743No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT30490.15011459223136062No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG29670.14607740083648638No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGTGA29490.14519118809127007No Hit
GTATATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTATCTATTA28960.14258178389702209No Hit
TTCCTATGCCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT28720.14140016690340035No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28010.13790454996393606No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA27400.13490127343848082No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26900.13243957136843557No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTACTGATGGTGAGAGT26150.12874701826336765No Hit
CAGTAATAGATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA25980.12791003955955227No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25780.12692535873153415No Hit
GTGTTACTAGCGACTACTTCGCCTGGTACCAACAGAAACCTGGCCAGGCT25750.12677765660733145No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25360.12485752899269614No Hit
ACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTA25220.12416825241308345No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGGAAT24350.1198848908112047No Hit
GTATTGACGCAGTCTCCAGGCTCCCTGTCTTTGTCTCCGGGGGAAAGAGC23750.11693084832715035No Hit
CTCCAGGCTCCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGC23700.11668467812014584No Hit
CTATTACTGTGCGAGAGATGAAGGTCGCCACGATGACTTAATTACTTATT23390.11515842283671776No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC23040.11343523138768609No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22900.1127459548080734No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCCGGGCCAGTCAGA22110.10885646553740187No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA22020.10841335916479372No Hit
CAGATATACTGTCTGCTTGGAGTTGTCTCTGGAGATGGTGAATCGGCCCC22000.10831489108199192No Hit
GAGTGTTACTAGCGACTACTTCGCCTGGTACCAACAGAAACCTGGCCAGG21990.108265657040591No Hit
CTACGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACTCCA21940.10801948683358649No Hit
GGGCTGGAGTGGGTGGCACTTATTTGGTATGATGGAGGAAATGCATACTA21230.1045238698941222No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20970.10324378481769865No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC20800.10240680611388327No Hit
GCCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC20680.1018159976170724No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG20610.10147135932726606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)