FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005108591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634696
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATATACTACGCAGACTCTGTTAAGGGCCGCTTCACCATCTCCAGAGA204820.7773951909442304No Hit
GTAATAAAGAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAAAGA117270.4450987893859481No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC107260.40710579133228275No Hit
GTATATGGTAGTGCCACTGTGACTAATGTATGAAATCCACTCCAGCCCCT105070.39879363691294933No Hit
CCTATGTGGTGACTCAGCCACCCTCAGTGTCCGTGGCCCCAGGAAAGACG96020.3644443229883068No Hit
CTTTATTACTGTGCGCGAGTGGGGAAAGGATACTCTGATAGTAGTGGCGG85200.3233769664507784No Hit
GACCTAAGACGGTCAATTTGGTCCCTCCGCCGAACACTCTCGGATGGTCA83030.3151407221174663No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA81230.30830881437554847No Hit
TTCTGATACCGACCGGCCCTCAGGAATTCCTGAGCGATTCTCTGGCTCCA79770.30276737809599286No Hit
GCCTTGGGCTGACCTAAGACGGTCAATTTGGTCCCTCCGCCGAACACTCT77020.2923297412680628No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA72420.27487042148316165No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT71820.27259311890252236No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT71220.27031581632188306No Hit
TTATTACTGTGCGCGAGTGGGGAAAGGATACTCTGATAGTAGTGGCGGTT70800.26872170451543553No Hit
CTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCAGTGTCCGTGGCCC65600.2489850821498951No Hit
CAGTAATAAAGAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAAA63820.24222908449399852No Hit
AACTAAAAGTGTCCACTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGG62970.23900290583809292No Hit
ATCCCACACCTGACAGAAGTAGTCGGCCTCATCCCCGGCTTCGACCCTGC62300.23645991795637902No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA61550.23361328973057993No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT60430.22936232491338657No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG59390.22541500044027848No Hit
GCACAGACTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCAGTGTCC58390.22161949613921303No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT58190.22086039527899992No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG58030.22025311459082947No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA57970.22002538433276553No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC55800.21178913999945348No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGGTGACTCAGCCAC55610.211067994182251No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC54470.20674111927903638No Hit
GTATCAGAATAGATGACCACCACAGGGGCCTGGCCTGGCCTCTGCTGGTA54320.20617179363387655No Hit
GGCCAGGCCCCTGTGGTGGTCATCTATTCTGATACCGACCGGCCCTCAGG54130.20545064781667413No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG53640.203590850709152No Hit
ATTCTGATACCGACCGGCCCTCAGGAATTCCTGAGCGATTCTCTGGCTCC53170.20180696368765125No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA51980.19729031356938337No Hit
ATCCGAGAGTGTTCGGCGGAGGGACCAAATTGACCGTCTTAGGTCAGCCC51880.19691076313927677No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA51580.19577211184895715No Hit
ATACTACGCAGACTCTGTTAAGGGCCGCTTCACCATCTCCAGAGACAACG51400.19508892107476536No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA50800.1928116184941261No Hit
GTGTGGGATAGTACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAA49170.18662494648338934No Hit
GGATAGTACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAAATTGA48760.18506878971995253No Hit
CTCTCGGATGGTCAGGAGTACTATCCCACACCTGACAGAAGTAGTCGGCC45480.17261953561245774No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT45470.1725815805694471No Hit
GTACTATCCCACACCTGACAGAAGTAGTCGGCCTCATCCCCGGCTTCGAC44850.1702283679027865No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGG44720.169734952343648No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG44710.16969699730063734No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44370.16840652583827506No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA44130.16749560480601935No Hit
CCCTCAGTGTCCGTGGCCCCAGGAAAGACGGCCAGGATTCCCTGTGGGGG43480.1650285270103268No Hit
CCCCTGTGGTGGTCATCTATTCTGATACCGACCGGCCCTCAGGAATTCCT43030.16332055007484736No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC42470.1611950676662507No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42050.1596009558598032No Hit
GACTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCAGTGTCCGTGGC41490.15747547345120652No Hit
GGCCAGGCCAGGCCCCTGTGGTGGTCATCTATTCTGATACCGACCGGCCC41460.15736160832217455No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA40730.15459089018239677No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC40650.15428724983831152No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGGTGGTCATCTATTCTGAT40090.15216176742971485No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG39970.151706306913587No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39810.15109902622541652No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA38970.14791080261252154No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC38390.14570941011790356No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC38200.1449882643007011No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT37810.14350801762328555No Hit
GCCATGGCCTGGACCTTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGA37600.14271096172006184No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA36750.13948478306415615No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT36400.13815635655878325No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG36340.13792862630071934No Hit
CATCTATTCTGATACCGACCGGCCCTCAGGAATTCCTGAGCGATTCTCTG36130.13713157039749557No Hit
CTTTTAGTTTCTATGTTGTCTCCCCCACAGGGAATCCTGGCCGTCTTTCC35600.13511995311793087No Hit
CCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGGACACTTTTAGTT35500.13474040268782433No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG35420.13443676234373908No Hit
CTCACTGCACAGACTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCA35090.1331842459243875No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34790.13204559463406784No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT34500.13094489838675885No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT34260.13003397735450314No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG34190.12976829205342855No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG34030.12916101136525807No Hit
GTGCTGGGTTTTCCTCGTTGCTATTTTAGAAGGTGTCCAATGCGAGGTGC33440.12692166382762946No Hit
GAATAGATGACCACCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTG33290.12635233818246963No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCTTTCTCCTCCTCGGCCTCCTCTC32940.1250239116770967No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGT32900.1248720915050541No Hit
GGCCTGGACCTTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG32760.12434072090290492No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA32240.12236705866635088No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC31770.1205831716448501No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC31530.1196722506125944No Hit
GTTAAGGGCCGCTTCACCATCTCCAGAGACAACGCCAAGAACTCACTCTT31460.1194065653115198No Hit
GTCTCTGGATTCACCTTCAGGATTTATGAGATGAATTGGGTCCGCCAGGC31340.11895110479539196No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC31310.11883723966635998No Hit
ACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAAATTGACCGTCTT30990.11762267829001904No Hit
CTGCACAGACTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCAGTGT30940.11743290307496576No Hit
TAGTACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAAATTGACCG30630.11625629674163547No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGG30630.11625629674163547No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC30160.1144724097201347No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG30150.11443445467712403No Hit
GTACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAAATTGACCGTC29990.11382717398895356No Hit
TACCAGCAGAGGCCAGGCCAGGCCCCTGTGGTGGTCATCTATTCTGATAC29480.11189146679541016No Hit
CTCTGATAGTAGTGGCGGTTTTGATTACTGGGGCCCGGGAACCCTGGTCA29190.11079077054810119No Hit
GCCTGGAGGGTCCCTGAGACTCTCGTGTGCAGTCTCTGGATTCACCTTCA28580.10847551292445123No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC28540.10832369275240862No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGAATTCCT28160.10688140111800376No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT28110.10669162590295046No Hit
GGCCTGGCCTGGCCTCTGCTGGTACCAGTGGACACTTTTAGTTTCTATGT27990.10623616538682262No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA27980.10619821034381195No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAC27940.10604639017176934No Hit
CTATCAGAGTATCCTTTCCCCACTCGCGCACAGTAATAAAGAGCCGTGTC27350.10380704263414071No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA27260.10346544724704483No Hit
TTTAGAAGGTGTCCAATGCGAGGTGCAGTTGGTGGAGTCCGGGGGAGGCT27000.10247861612876781No Hit
ACCTAAGACGGTCAATTTGGTCCCTCCGCCGAACACTCTCGGATGGTCAG26800.1017195152685547No Hit
TATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGT26660.10118814466640554No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCTTTCTCCTCCTCGGCCTC26660.10118814466640554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)