FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2634696
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATATACTACGCAGACTCTGTTAAGGGCCGCTTCACCATCTCCAGAGA187770.712681842611064No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC103510.3928726502032872No Hit
GTAATAAAGAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAAAGA102310.3883180450420087No Hit
GTATATGGTAGTGCCACTGTGACTAATGTATGAAATCCACTCCAGCCCCT93380.3544241916334939No Hit
CCTATGTGGTGACTCAGCCACCCTCAGTGTCCGTGGCCCCAGGAAAGACG87980.33392846840774043No Hit
GACCTAAGACGGTCAATTTGGTCCCTCCGCCGAACACTCTCGGATGGTCA81030.3075497135153354No Hit
GCCTTGGGCTGACCTAAGACGGTCAATTTGGTCCCTCCGCCGAACACTCT76910.29191223579494563No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA74620.28322053094550564No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT73660.2795768468164828No Hit
CTTTATTACTGTGCGCGAGTGGGGAAAGGATACTCTGATAGTAGTGGCGG72730.27604702781649193No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC70130.2661787166337217No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT66300.25164193516064093No Hit
TTCTGATACCGACCGGCCCTCAGGAATTCCTGAGCGATTCTCTGGCTCCA65760.24959236283806557No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA64170.24355751099937148No Hit
CTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCAGTGTCCGTGGCCC61130.23201917792413243No Hit
TTATTACTGTGCGCGAGTGGGGAAAGGATACTCTGATAGTAGTGGCGGTT59910.22738866267683253No Hit
AACTAAAAGTGTCCACTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGG58790.22313769785963922No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT58060.2203669797198614No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA57840.21953196877362702No Hit
CAGTAATAAAGAGCCGTGTCGTCGACTCTCAGGCTGTTCATTTGCAGAAA57160.21695102584890247No Hit
ATCCCACACCTGACAGAAGTAGTCGGCCTCATCCCCGGCTTCGACCCTGC57070.21660943046180658No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56800.21558464430051894No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGGTGACTCAGCCAC55320.209967297934942No Hit
ATCCGAGAGTGTTCGGCGGAGGGACCAAATTGACCGTCTTAGGTCAGCCC54550.20704475962312158No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT53880.20450177174140774No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA52610.19968148127905458No Hit
GCACAGACTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCAGTGTCC52190.19808736947260708No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT51480.1953925614188506No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG51410.19512687611777602No Hit
GTATCAGAATAGATGACCACCACAGGGGCCTGGCCTGGCCTCTGCTGGTA50560.19190069746187036No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA50480.19159705711778513No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA49990.18973726001026303No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG49650.18844678854790078No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC47600.18066600473071656No Hit
ATTCTGATACCGACCGGCCCTCAGGAATTCCTGAGCGATTCTCTGGCTCC46500.17649094999954454No Hit
ATACTACGCAGACTCTGTTAAGGGCCGCTTCACCATCTCCAGAGACAACG45320.1720122549242873No Hit
GTGTGGGATAGTACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAA44630.16939335695655208No Hit
GGCCAGGCCCCTGTGGTGGTCATCTATTCTGATACCGACCGGCCCTCAGG44200.16776129010709395No Hit
GTACTATCCCACACCTGACAGAAGTAGTCGGCCTCATCCCCGGCTTCGAC44130.16749560480601935No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT42470.1611950676662507No Hit
GGATAGTACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAAATTGA42360.1607775621931335No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC41970.15929731551571794No Hit
CTCTCGGATGGTCAGGAGTACTATCCCACACCTGACAGAAGTAGTCGGCC41200.15637477720389753No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA40840.15500839565551397No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGG40600.15409747462325826No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA40460.15356610402110907No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC40300.15295882333293861No Hit
CCCTCAGTGTCCGTGGCCCCAGGAAAGACGGCCAGGATTCCCTGTGGGGG39680.150605610666278No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA38640.14665828619316992No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG38260.14521599455876502No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC38140.14476053404263717No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT38130.1447225789996265No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA37770.14335619745124295No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA37490.14229345624694462No Hit
GACTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCAGTGTCCGTGGC37360.1418000406878061No Hit
CTTTTAGTTTCTATGTTGTCTCCCCCACAGGGAATCCTGGCCGTCTTTCC36710.13933296289211355No Hit
CCCCTGTGGTGGTCATCTATTCTGATACCGACCGGCCCTCAGGAATTCCT36640.13906727759103896No Hit
GCCATGGCCTGGACCTTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGA36410.1381943116017939No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG36080.13694179518244232No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC35790.1358410989351333No Hit
GGCCAGGCCAGGCCCCTGTGGTGGTCATCTATTCTGATACCGACCGGCCC35330.1340951669566432No Hit
CCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGGACACTTTTAGTT34760.13193172950503587No Hit
CTCACTGCACAGACTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCA34710.1317419542899826No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGGTGGTCATCTATTCTGAT34560.1311726286448228No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG34530.13105876351579082No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT34220.12988215718246052No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA34210.12984420213944986No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG34080.12935078658031135No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG34080.12935078658031135No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT33280.12631438313945897No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT32390.1229363843115107No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT32360.12282251918247873No Hit
CATCTATTCTGATACCGACCGGCCCTCAGGAATTCCTGAGCGATTCTCTG32210.12225319353731891No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA32000.12145613763409517No Hit
GAATAGATGACCACCACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTG31900.12107658720398862No Hit
CTGCACAGACTCTGTGACCTCCTATGTGGTGACTCAGCCACCCTCAGTGT31850.12088681198893535No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31730.1204313514728075No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC30630.11625629674163547No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC30570.11602856648357154No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT30550.11595265639755023No Hit
GTGCTGGGTTTTCCTCGTTGCTATTTTAGAAGGTGTCCAATGCGAGGTGC30320.11507969040830517No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGT30260.11485196015024125No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCTTTCTCCTCCTCGGCCTCCTCTC30240.11477605006421994No Hit
GTCTCTGGATTCACCTTCAGGATTTATGAGATGAATTGGGTCCGCCAGGC30130.11435854459110271No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA29830.1132198933007831No Hit
TAGTACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAAATTGACCG28940.10984189447283482No Hit
ACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAAATTGACCGTCTT28660.10877915326853649No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC28380.10771641206423815No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28370.10767845702122748No Hit
GGCCTGGACCTTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG28010.10631207547284394No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC27590.10471796366639642No Hit
GTACTCCTGACCATCCGAGAGTGTTCGGCGGAGGGACCAAATTGACCGTC27250.10342749220403416No Hit
GTTAAGGGCCGCTTCACCATCTCCAGAGACAACGCCAAGAACTCACTCTT27210.10327567203199153No Hit
CTCTGATAGTAGTGGCGGTTTTGATTACTGGGGCCCGGGAACCCTGGTCA26850.10190929048360799No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26720.10141587492446948No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG26610.10099836945135225No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26560.10080859423629898No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGG26540.10073268415027768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)