FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497222
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT87860.35183095455670343No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC82570.33064741540800135No Hit
GTACTACTGTCAGCAGTATCGAAGCTCACTTCCGTACACTTTTGGCCAGG68490.27426476300465075No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG54680.21896331203233033No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC53720.2151190402775564No Hit
CAGTAGTACACTGCAAAATCTTCAGCCTCCAGTTTGCTGATGGTGAGAGT43860.17563516579623278No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42420.16986875816407193No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAAACTGGAGGCTGAAGATT42130.16870746773815062No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA41710.16702559884543705No Hit
ATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT41500.16618466439908025No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41360.1656240414348424No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC38530.1542914486577485No Hit
AGGAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA36690.1469232611277652No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36100.14456063577847705No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC36010.144200235301467No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35500.14215796593174337No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34780.13927476211566292No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT34330.13747275973061265No Hit
GTATAGCAGTGGCTATTCCGGGTACGGTCTGGACGTCTGGGGCCAGGGGA34150.13675195877659255No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA34080.1364716472944736No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA33640.13470968940686892No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT33360.1335884434783932No Hit
GTGTACTACTGTCAGCAGTATCGAAGCTCACTTCCGTACACTTTTGGCCA33250.13314795400649201No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC32460.12998443870829265No Hit
GAAATACACAGCCGTGTCGTCAGCTCTCAGGCTGCTCATTTGCAGATACA32370.1296240382312826No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32020.12822248082068793No Hit
GTGTATTTCTGTGCGAAAGATCTCGTCGCCGGGTATAGCAGTGGCTATTC31640.1267007899177566No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTTCTTAGCCTGGTACC31460.12597998896373652No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA30680.12285651816298271No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG29800.1193326023877733No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT29550.11833148995163424No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG29380.1176507334950597No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29020.11620913158701948No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28760.1151679746534349No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA28590.11448721819686035No Hit
GGGCTGGAGTGGGTGACATTTATTTCATATGAGGGAAGTAGGAAATACTA27620.11060290194464088No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC27180.10884094405703618No Hit
TCCATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC26760.1071590751643226No Hit
CTGCTATACCCGGCGACGAGATCTTTCGCACAGAAATACACAGCCGTGTC26450.10591769574351019No Hit
TTCTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCAT26140.10467631632269778No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA26110.1045561828303611No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT25480.10203337949129072No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC25350.10151280102449842No Hit
GTAGTACACTGCAAAATCTTCAGCCTCCAGTTTGCTGATGGTGAGAGTGA25260.10115240054748838No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT25240.10107231155259726No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)