FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005108608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497222
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112340.449859884303438No Hit
CTCCTGGGAGTTACCCGATTGGAGGACGTTATCCACCTTCCACTGTACTT88970.3562758937731607No Hit
GTACTACTGTCAGCAGTATCGAAGCTCACTTCCGTACACTTTTGGCCAGG68440.27406454051742296No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65490.2622514137709823No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG49720.19910124129933182No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT48140.1927742107029331No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC47200.1890100279430503No Hit
CAGTAGTACACTGCAAAATCTTCAGCCTCCAGTTTGCTGATGGTGAGAGT45250.18120135094116582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41860.16762626630712046No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC41410.1658242639220702No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAAACTGGAGGCTGAAGATT40040.16033816777202828No Hit
ATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT36900.14776419557412196No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA36660.14680312763542847No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA35670.1428387223883179No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC35150.1407564085211487No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA34820.13943494010544516No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGTCCTCCAATCG33680.13486986739665116No Hit
GTGTACTACTGTCAGCAGTATCGAAGCTCACTTCCGTACACTTTTGGCCA33500.13414906644263105No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32790.1313059071239962No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32730.13106564013932281No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32430.129864305215956No Hit
GAAATACACAGCCGTGTCGTCAGCTCTCAGGCTGCTCATTTGCAGATACA32210.12898332627215361No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGACGTTAT31680.12686096790753887No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31470.12602003346118207No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA31340.12549945499438978No Hit
CTGCTATACCCGGCGACGAGATCTTTCGCACAGAAATACACAGCCGTGTC31080.12445829806080518No Hit
AGGAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA30570.12241602869108154No Hit
GTATAGCAGTGGCTATTCCGGGTACGGTCTGGACGTCTGGGGCCAGGGGA29100.11652948756658399No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT29080.11644939857169286No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTTCTTAGCCTGGTACC28360.11356619475561243No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC27990.11208454835012667No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT27730.11104339141654206No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA27560.11036263495996751No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27560.11036263495996751No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT27500.11012236797529415No Hit
GTAGTACACTGCAAAATCTTCAGCCTCCAGTTTGCTGATGGTGAGAGTGA27040.10828032109279832No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26310.10535707277927232No Hit
GTGTATTTCTGTGCGAAAGATCTCGTCGCCGGGTATAGCAGTGGCTATTC26250.10511680579459895No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA25880.10363515938911318No Hit
TCCATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC25580.10243382446574634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)