FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005108677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005108677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677267
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82091.2120773638756945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.6134951208312232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40920.6041930287464176No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA36970.54587038789724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30990.45757433921924434No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA30080.44413798398563636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG29720.43882250279431895No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCG22190.32764035454259544TruSeq Adapter, Index 6 (96% over 33bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA17060.2518947475663217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG16940.2501229205025492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16770.24761283216220487No Hit
TATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA16490.24347856901340237TruSeq Adapter, Index 16 (97% over 34bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG15800.2332905633967106No Hit
GTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCACC14850.2192635991418451Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA14660.21645820629087198No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG13880.20494133037635082No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA13830.20420306909977895No Hit
GTATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCC13350.19711576084468904TruSeq Adapter, Index 1 (96% over 32bp)
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13300.19637749956811715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT13060.19283384544057217No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA12950.19120967063211408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG12620.18633714620673975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT12060.1780686199091348No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT11930.17614914059004794No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA11220.1656658304627274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10970.16197452407986807No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA10600.15651139063323624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10580.15621608612260748No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT10410.15370599778226313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10230.1510482571866044No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10170.15016234365471814No Hit
TATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGG10130.14957173463346066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT9840.1452898192293438No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA9600.14174616510179885No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT9490.14012199029334071TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCCG8850.1306722459532208TruSeq Adapter, Index 1 (96% over 33bp)
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA8220.12137015386841526No Hit
GGTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCC8190.12092719710247214TruSeq Adapter, Index 6 (96% over 32bp)
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA7660.11310162757081034No Hit
GAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCA7420.10955797344326536Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCGCGTATGCCGT7310.10793379863480726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT7240.10690023284760664No Hit
GTATCAACGCAGAGTACGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA7060.10424249225194791TruSeq Adapter, Index 16 (97% over 37bp)
GTATCAACGCAGAGTACGGGGTATCAACGCAGAAAAAAAAAAAAAAAAAA6990.10320892646474729No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAA6950.10261831744348979No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA6780.10010822910314543No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)