FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005109071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970911
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC95800.48606963987719387No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC69650.3533898790965193No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT69430.35227364401538175No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA40340.20467692351405012No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39910.20249519131000843No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGAATTAGCAG38830.19701549182078743No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34670.17590850119564No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33800.17149429882932307No Hit
GTAAAACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGAGA32760.16621755117303622No Hit
GTGTTTTACTGTGCGCGAGCACGGTCGGGGTTAGGCGCTACTCATGGTGC32740.16611607525656916No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32570.1652535299665992No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32000.16236146634728812No Hit
GAGTTACAGTACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCA31790.16129596922438405No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30370.15409117915522313No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG30120.15282273019938494No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29710.15074247391181034No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29390.14911885924833745No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27970.14191406917917654No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27730.14069635818157186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT27050.137246177021692No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA26990.13694174927229083No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26070.1322738571148063No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC25950.13166500161600397No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25650.13014286286899815No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT25420.12897588982962702No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25330.12851924820552527No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24880.12623604008501652No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24830.12598235029384888No Hit
GTTTTACTGTGCGCGAGCACGGTCGGGGTTAGGCGCTACTCATGGTGCTT24670.12517054296211244No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT24280.12319176259100488No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23920.1213651960945979No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22920.11629140027124513No Hit
CAGTAAAACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAACTTCAGGGA22180.11253679136196408No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTC22120.1122323636125629No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC22080.11202941177962882No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21400.10857923061974893No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCC21110.10710782983097665No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20720.10512904945986906No Hit
ACAGTACCCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA20630.10467240783576731No Hit
CCATATCAGTAGACACGTCCAAGAAGCAGTTCTCCCTGAAGTTGAGCTCT20370.1033532209216956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)