FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005109108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480388
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT84990.3426480050701745No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATATAGG75880.3059198802767954No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC74640.30092066241249354No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68980.2781016518383414No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG65420.2637490586150231No Hit
GCCTATATGGAGCTGAGCAGGCTGAGATCTGACGACACGGCCGTGTATTA55800.22496480389358442No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT55750.22476322252808836No Hit
CCCTAATAGTGGTGGCACAAACTATACACAGAAGTTTCAGGGCAGGGTCA52970.21355529860650835No Hit
CTATTAGGGTTGATCCATCCCATCCACTCAAGCCCTTGTCCAGGGGCCTG52820.21295055451002023No Hit
CCATATAGGCTGTGCTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCC49370.1990414402907932No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC47350.19089755312475307No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG44150.17799634573300627No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT43480.17529515543535928No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA42390.17090068166754557No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC40900.16489355697576347No Hit
GTGTATTACTGTGTGAGAGCGGCCCGGGGCAGTGGCTGGTTCCATGATGT40820.1645710267909698No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA40270.16235363177051335No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40140.16182952022022362No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA40080.16158762258162837No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39690.1600152879307592No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39270.15832200446059247No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39140.15779789291030275No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38710.15606429316703677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36850.14856546637058396No Hit
ACTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATG35500.14312276950219077No Hit
GAGTTACAGTACCCCCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA35410.1427599230442979No Hit
ATATGGAGCTGAGCAGGCTGAGATCTGACGACACGGCCGTGTATTACTGT35320.14239707658640502No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34130.1375994400875992No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGCCTGCTCAGCTCCATATA34030.1371962773566071No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT33690.13582552407123402No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG33690.13582552407123402No Hit
GGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAAC33260.13409192432796804No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT32530.1311488363917258No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC32210.12985871565255114No Hit
CATATAGGCTGTGCTGATGGACGTGTCCCTGGTCATGGTGACCCTGCCCT32070.12929428782916222No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCCAGGTTCAGTG32060.129253971556063No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA31890.12856859491337647No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30440.12272273531399121No Hit
GTATTACTGTGTGAGAGCGGCCCGGGGCAGTGGCTGGTTCCATGATGTTT30300.12215830749060229No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29990.1209085030245268No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT29630.1194571171929553No Hit
GTGCATATAGTAGCCGGTGAAGATGTATCCAGAGGCCTTGCAGGAGACCT29120.11740098726489565No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29030.11703814080700277No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATC28830.1162318153450186No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT28560.11514327597133996No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG28510.11494169460584391No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27760.11191797412340328No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC27390.11042627201873255No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT27330.11018437438013731No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA26780.10796697935968082No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT26190.10558831924682752No Hit
ACTATACACAGAAGTTTCAGGGCAGGGTCACCATGACCAGGGACACGTCC25980.10474167751174414No Hit
GTGTGAGAGCGGCCCGGGGCAGTGGCTGGTTCCATGATGTTTTTGACATC25940.1045804124193473No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA25680.10353218931876786No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC24860.10022625492463275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)