FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005109181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429778
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58571.362796606620162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.6936139122989078No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA29210.6796532163116772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26360.6133399103723317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG23530.547491960965894No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA21960.5109614731326406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG21330.4963027423460485No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA18380.42766265374216456No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA16180.37647343512231896No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13660.31783851197595037No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA12780.2973628245280121No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12590.2929419374653891No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA12430.28921908520212763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11350.2640898324251125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11290.2626937628263894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT11000.255946093099228No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA10940.25455002350050493No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10800.2512925277701511No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA10510.24454485804298962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10420.24245075364490504No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT10210.23756451004937432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10140.2359357621841974No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10130.23570308391774358No Hit
GTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCACC9650.2245345271279591Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT9280.21592543126916688RNA PCR Primer, Index 47 (95% over 22bp)
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA9110.21196990073945152No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG8880.2066183006110131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT8720.2028954483477516No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA8340.19405367422250558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT8200.19079617849215177No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA7480.17404334330747503Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7390.17194923890939043No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT7370.17148388237648274RNA PCR Primer, Index 47 (95% over 24bp)
TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA7330.17055316931066739No Hit
GTATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA7310.17008781277775967No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA7250.16869174317903662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6980.16240942998478283No Hit
GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAA6800.15822122118861365No Hit
GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA6740.1568251515898906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG6600.15356765585953677No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6450.15007748186272912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT6430.14961212532982143No Hit
TATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGG6430.14961212532982143No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCG6410.14914676879691377TruSeq Adapter, Index 6 (96% over 33bp)
GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6350.1477506991981907No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA6320.14705266439882916No Hit
GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6210.14449320346783687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT6190.1440278469349292No Hit
GTATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCC6070.14123570773748306TruSeq Adapter, Index 1 (96% over 32bp)
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6000.13960695987230615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT5870.13658214240840622No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA5850.1361167858754985No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGT5570.1296017944147909No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCGCGTATGCCGTCTT5560.12936911614833704No Hit
GAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5500.127973046549614No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA5380.12518090735216786Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5240.12192341162181405No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA5050.11750252455919102No Hit
TATCAACGCAGAGTACGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4880.11354699402947568No Hit
GTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAA4730.11005682003266803No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAGCAGAAGACGGCATA4700.1093587852333065No Hit
TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA4680.10889342870039881No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC4660.10842807216749112Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG4560.1061012895029527Clontech Universal Primer Mix Long (96% over 25bp)
GTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4480.10423986337132195No Hit
GAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCA4470.1040071851048681Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC4440.10330915030550655Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4420.10284379377259888No Hit
GTATCAACGCAGAGTACGGGAAGCAGCGGTATCAACGCAAAAAAAAAAAA4400.1023784372396912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4340.10098236764096813No Hit
GGTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA4320.10051701110806044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)