FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005109194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518322
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA70040.46129872319573845No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT55720.3669840784761072No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT45000.29637981930051727No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATACA43470.2863029054442997No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39300.25883837552245176No Hit
GATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCG33700.22195555356505406No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT30810.20292138294775416No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT26310.17328340101770243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25580.16847546172682737No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC25400.1672899424496253No Hit
TTATAGATCAGAGCCTTAGGGACTTTCCCTGATTTCTGCTGAAACCAGGC24430.1609013107891475No Hit
GTGTATTACTGTGCGAAAGATGGGAGCTCAAGGTCCCTTGTCTCCTGGGG23490.15471026567487No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC22670.14930956674539392No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT22310.1469385281909898No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTTGAGTCTGGAGGAGGCT22040.14516024927518667No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21760.1433161081773168No Hit
CAGTATAATAGTTTCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAAT20180.13290988341076532No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19070.12559918120135252No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18360.12092296627461104No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18320.12065951754634394No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGATAGAGTCACCATCACTTGCCG17940.11815675462780621No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA17910.11795916808160586No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17530.11545640516306818No Hit
GTCTGCATCTGTAGGAGATAGAGTCACCATCACTTGCCGGGCCAGTCAGA17250.1136122640651983No Hit
GTAGGAGATAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTGT17250.1136122640651983No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16450.10834328949985575No Hit
ATACTACGCAGACTCCGTAAAGGGCCGATTCACCATCTCCAGAGACAATT16300.10735535676885403No Hit
GTGATAACACATACTACGCAGACTCCGTAAAGGGCCGATTCACCATCTCC16120.10616983749165197No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC15940.10498431821444991No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15910.10478673166824956No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15750.10373293675518104No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15750.10373293675518104No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15400.10142776038284369No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)