FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005109282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702579
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144572.057704542834329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90161.2832720590851705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47430.6750842254038336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43000.6120308178866718No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG34650.49318297301798086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG32790.4667090818256737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG28700.408494987752267No Hit
TATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGG24330.34629557672517963No Hit
GAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGGAAGAGCACAC22900.3259419937117392No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA17620.2507903025851897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG16930.2409693429493338No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16340.23257171079693528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG15710.22360474765115382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG15350.21848076871070726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15250.2170574412272499No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAGCAGAAGACGGCATA15220.21663044298221268No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA13910.19798485294892104No Hit
GGTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATC13720.19528053073035204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT13000.18503257284945893No Hit
GTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGGAAGAGCACACGT12090.17208029274999678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA12070.17179562725330533No Hit
CTGGAATTCTGCTGAAAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG12010.1709416307632309Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11820.16823730854466187No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10200.1451794033126524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT10130.14418307407423223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT9950.14162108460400896No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9860.1403400898688973No Hit
GTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAA9550.13592777467017944No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9380.13350811794830192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG9140.1300921319880042No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAGCAGAAGACGGCATAC8960.12753014251778091No Hit
GAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8880.12639148053101504No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8570.12197916533229715No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8380.11927484311372814No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA8360.11899017761703666Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA8240.1172821846368878No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA8170.11628585539846764No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT8070.11486252791501027No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7870.11201587294809552No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGATATCAAACGTAAGTACATCTG7490.10660722851095748No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA7320.10418757178907993No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCCAAAAAAAAAAAAAAAAAAAA7060.10048692033209077TruSeq Adapter, Index 2 (96% over 31bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT7060.10048692033209077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)