FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005109343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082173
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159421.4731470846158607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78580.7261315889418789No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71770.6632026487447016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG60030.554717221738114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG52670.4867059148583452No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA41590.38431932787086726No Hit
GTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCACC41290.381547127862181Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCG39700.366854467816144No Hit
GTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCG39010.3604784077961657TruSeq Adapter, Index 6 (96% over 33bp)
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA31710.29302154091813415No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG29280.2705667208477757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG29140.26927302751038884No Hit
TATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGG29050.26844136750778297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG28930.2673324875043085No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT27840.25726016080608183No Hit
TATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA27420.25337908079392113TruSeq Adapter, Index 16 (97% over 34bp)
GTATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCC25800.23840920074701552TruSeq Adapter, Index 1 (96% over 32bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24560.22695077404444575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT23220.21456828067231395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT21140.19534769394542278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA19960.18444370724459028No Hit
GAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAGTCA19730.1823183539045975Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA19410.17936134056199887No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA19280.17816005389156817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG18400.1700282671994219No Hit
TATCAACGCAGAGTACGGGTGTGACTGGAGTTCAGACGTGTGCTCTTCCG17920.16559274718552394TruSeq Adapter, Index 1 (96% over 33bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG17510.16180407384031945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT17150.15847743382989596No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA17090.15792299382815872No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16700.15431913381686663No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCGCGTATGCCGT16390.15145452714122418No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA16070.14849751379862552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT15920.14711141379428241No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT15380.14212145377864724RNA PCR Primer, Index 29 (95% over 23bp)
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAA14980.13842518710039892No Hit
CTGGAGTTCAGACGTGTGCTCTTCCGATCTCCCTTTCAGCAGAATTCCAG14690.1357453937586689Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA14360.13269597374911407No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA14300.1321415337473768No Hit
GGTATCAACGCAGAGTACGGGGTGACTGGAGTTCAGACGTGTGCTCTTCC14040.12973896040651542TruSeq Adapter, Index 6 (96% over 32bp)
GGTATCAACGCAGAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATC13940.12881489373695335No Hit
GTATCAACGCAGAGTACGGGTGACTGGAGTTCAGACGTGTGCTCTTCCGA13730.12687435373087297TruSeq Adapter, Index 16 (97% over 37bp)
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA13520.12493381372479262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT13340.12327049371958089No Hit
GTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAAAA12860.11883497370568293No Hit
GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAA12200.11273613368657323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT11610.10728414033615698No Hit
CGTCTGAACTCCAGTCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11600.10719173366920078TruSeq Adapter, Index 2 (95% over 21bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAA11460.10589804033181385No Hit
GAGTACGGGAAGCAGTGGTATCAACCCAAAAAAAAAAAAAAAAAAAAAAA11370.10506638032920798No Hit
GAGTACGGGGTGACTGGAATTCTGCTGAAAGGGAGATCGGAAGAGCACAC11210.10358787365790867No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)