FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005109421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240285
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGA111650.4983740907964835No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC70520.31478137826214075No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC66290.29589985202775543No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT64330.28715096516737826No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT61250.2734027143867856No Hit
CTCCAGGGACAGCGCCAAGAACTCACTGTATCTGCAAATGAAGAGCCTGA59590.2659929428621805No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTCCCTCTATGTCTT56400.25175368312513813No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGATACA55970.24983428447719822No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC55190.246352584604191No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC53920.24068366301608946No Hit
CATTAGTAGTGGTGGGGGTTCCATATACTACGCAGACTCTGTGAAGGGCC53110.23706805160950503No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC52900.23613067087446463No Hit
GTGTATTACTGTGCGAGAGATAAGCGAAGGAGACACTATGATAGTAGTGG52590.23474691836083356No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA52070.23242578511216208No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA49390.2204630214459321No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT49370.22037374709021396No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA47980.21416917936780364No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGAGGGAAGTGC46920.20943763851474254No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCT45300.2022064157015737No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGAGGGAAGTG44980.20077802601008352No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44270.19760878638208976No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA43990.1963589454020359No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA43430.19385926344192814No Hit
CTATGATAGTAGTGGTTACCACTTTGACTACTGGGGCCAGGGAACCCTGG43270.19314506859618308No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC43130.19252014810615614No Hit
TAGTAGCACTTCCCTCTATGTCTTCGGAACTGGGACCAAGGTCACCGTCC43000.19193986479398825No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41130.18359271253434273No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA40690.18162867670854377No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC40360.18015564983919458No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG39460.17613830383187853No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG39390.17582584358686507No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACTGTCAGGGAGG38790.17314761291532105No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG38100.17006764764304544No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38090.17002301046518634No Hit
TATAAAAGGTGTCCACTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT36970.16502364654497084No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG35480.15837270704396986No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT34760.15515883023811702No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATA33710.15047192656291497No Hit
GTATATGGAACCCCCACCACTACTAATGTATGAAACCCACTCCAGCCCCT33140.14792760742494818No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT32270.14404417295120933No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT32220.14382098706191399No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAGCG31840.14212477430326945No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC30910.1379735167623762No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29980.13382225922148297No Hit
CTATCATAGTGTCTCCTTCGCTTATCTCTCGCACAGTAATACACGGCCGT29940.13364371051004673No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA29280.1306976567713483No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC28360.12659103640831412No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG28150.12565365567327372No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG28120.12551974413969652No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC28080.12534119542826025No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT26990.12047574304162195No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG26970.1203864686859038No Hit
GTCCCTGGAGATGGTGAATCGGCCCTTCACAGAGTCTGCGTAGTATATGG26850.11985082255159499No Hit
GCTCATATGCAGGTAGTAGCACTTCCCTCTATGTCTTCGGAACTGGGACC26510.11833315850438672No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG25970.1159227508999971No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG25800.11516391887639295No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT25790.11511928169853389No Hit
GGTAGTAGCACTTCCCTCTATGTCTTCGGAACTGGGACCAAGGTCACCGT25710.11476218427566136No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGC25460.11364625482918468No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25360.113199883050594No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA24530.10949499728829144No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG24280.10837906784181477No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCC24080.10748632428463344No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24080.10748632428463344No Hit
GTATTACTGTGCGAGAGATAAGCGAAGGAGACACTATGATAGTAGTGGTT23830.10637039483815676No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCC23610.10538837692525727No Hit
GTGCTACTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGC23610.10538837692525727No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA22820.10186203987439099No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT22710.10137103091794125No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)