FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005109422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2240285
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGA107200.4785105466491986No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC62210.27768788346125606No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT61200.2731795284974903No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT60630.27063520935952345No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGATACA59180.2641628185699587No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC58780.26237733145559605No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTCCCTCTATGTCTT57800.2580028880254075No Hit
CTCCAGGGACAGCGCCAAGAACTCACTGTATCTGCAAATGAAGAGCCTGA55750.24885226656429874No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC54830.24474564620126457No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC53770.24001410534820344No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT52600.2347915555386926No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA50330.22465891616468442No Hit
CATTAGTAGTGGTGGGGGTTCCATATACTACGCAGACTCTGTGAAGGGCC49840.2224716944495901No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC48010.21430309090138083No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA46020.20542029250742652No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA45030.20100121189937886No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG44950.20064411447650635No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA44910.20046556576507005No Hit
GTGTATTACTGTGCGAGAGATAAGCGAAGGAGACACTATGATAGTAGTGG44520.19872471582856646No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCT44470.19850152993927112No Hit
CTATGATAGTAGTGGTTACCACTTTGACTACTGGGGCCAGGGAACCCTGG41840.1867619521623365No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGAGGGAAGTGC41340.18453009326938313No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41100.18345880100076553No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA40960.18283388051073857No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC40700.18167331388640284No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC40310.17993246394989923No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40020.17863798579198628No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG39880.17801306530195934No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGAGGGAAGTG39820.17774524223480495No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT39200.17497773720754278No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG38870.1735047103381936No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37340.1666752221257563No Hit
TAGTAGCACTTCCCTCTATGTCTTCGGAACTGGGACCAAGGTCACCGTCC36950.1649343721892527No Hit
TATAAAAGGTGTCCACTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT36560.1631935222527491No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT34730.15502491870453983No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGACTGTCAGGGAGG32750.14618675748844456No Hit
CTATCATAGTGTCTCCTTCGCTTATCTCTCGCACAGTAATACACGGCCGT31540.14078565896749742No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA31410.14020537565532956No Hit
ATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAGCG30300.13525064891297314No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC30270.13511673737939592No Hit
GTATATGGAACCCCCACCACTACTAATGTATGAAACCCACTCCAGCCCCT30140.13453645406722806No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA29770.13288487848644256No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATA29470.13154576315067057No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29030.1295817273248716No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG28660.12793015174408612No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG28430.1269034966533276No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCC28340.12650176205259597No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG28300.12632321334115973No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC28100.12543046978397837No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG28080.12534119542826025No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT28040.12516264671682398No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27260.12168094684381675No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA27110.12101138917593073No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG26880.1199847340851722No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC26820.1197169110180178No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT26220.11703868034647377No Hit
GTCCCTGGAGATGGTGAATCGGCCCTTCACAGAGTCTGCGTAGTATATGG25560.11409262660777536No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT25330.1130659715170168No Hit
ATCATAGTGTCTCCTTCGCTTATCTCTCGCACAGTAATACACGGCCGTGT23880.10659358072745209No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG23740.10596866023742514No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG23670.10565619999241167No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA23460.10471881925737127No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA23320.10409389876734434No Hit
GGTAGTAGCACTTCCCTCTATGTCTTCGGAACTGGGACCAAGGTCACCGT23280.10391535005590806No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCT23070.10297796932086767No Hit
TCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGAC23050.10288869496514952No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT22780.1016834911629547No Hit
CCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC22720.10141566809580031No Hit
CTCCCTGACAGTCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACT22580.10079074760577338No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC22530.10056756171647804No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAACTGCT22510.10047828736075991No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGC22460.10025510147146457No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)