FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005109449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041536
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT67650.22242051384563588No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT58250.1915150765928794RNA PCR Primer, Index 17 (95% over 21bp)
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA41170.1353592395421261No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC38740.12736985523104116No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT37890.1245752146283983RNA PCR Primer, Index 17 (95% over 23bp)
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT37430.12306282089049744No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA36710.12069559590943524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33720.1108650366130797No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG32040.1053415116572679No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT31850.10471682728726538No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)