FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005109561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225251
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTACTGTGCGAAAGGCCTGTGGGAGAATTCCTACACTGACACCCC89660.40292083904242715No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC57430.25808324544062666No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATGGGGGCTGATTGA55590.2498145153063632No Hit
GTAATATATGGCCGTGTCCGCGACTCTCAGGCTGCTCATTTGCAGAAACA50090.22509820240503206No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA49340.22172779610030507No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT47410.21305461720947436No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGAAACATTGTCGAAA46790.21026841466423338No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCCATGAGCTGACACAGCCA46740.2100437209105849No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCCATGAGCTGACACAGCCACCCTCG46010.20676319210731733No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA45030.20235919453580742No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42860.19260748562746405No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA41040.18442863299465992No Hit
GGCTCTGAGGCCTCCCATGAGCTGACACAGCCACCCTCGATGTCGGTGTC40930.1839343067366333No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40590.18240638921182376No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC40290.18105822668993296No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT39230.1762947191125855No Hit
CATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC39160.17598014785747765No Hit
GTAGTAGTCAGCCTCGTCTTCTGCCTGGACTCCAGTGATGGTCAAGGTGA38110.1712615790308599No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA37150.16694745896080937No Hit
TCACTGGAGTCCAGGCAGAAGACGAGGCTGACTACTACTGTCAATCAGCC36470.16389162391119025No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC36030.16191431887908375No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA35940.1615098701225165No Hit
ACAGAAGGCAGGGCAGGCCCCTGTTTTGGTCGTGCATAAAGACAGTGAGA35420.1591730550845725No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG34690.1558925262813049No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACATGGG33540.15072456994739022No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCCAT33310.14969097868060727No Hit
ATATTACTGTGCGAAAGGCCTGTGGGAGAATTCCTACACTGACACCCCCG32510.14609587862223183No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG32030.14393881858720656No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA31470.14142224854634375No Hit
GACTACTACTGTCAATCAGCCCCCATGTTCGGCGGAGGGACCAAGCTGAC31340.14083804478685777No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA30530.13719800597775264No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC30490.13701825097483386No Hit
GCCATATATTACTGTGCGAAAGGCCTGTGGGAGAATTCCTACACTGACAC30370.13647898596607755No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA30340.13634416971388846No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT30170.13558021095148368No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30070.13513082344418675No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30020.13490612969053828No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29380.13203004964383794No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC29040.13050213211902836No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28700.1289742145942188No Hit
GTATTACGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT28680.12888433709275943No Hit
TTCCTACACTGACACCCCCGCCTACAAGTATTACGGTGTGGACGTCTGGG28500.12807543957962494No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC28460.1278956845767062No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG27840.1251094820314652No Hit
GTTTTGGTCGTGCATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG27670.12434552326906043No Hit
GTCTTTATGCACGACCAAAACAGGGGCCTGCCCTGCCTTCTGTTGGTACC27530.12371638075884474No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA26200.11773952691179557No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG25270.11356022309393413No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA23980.10776312424980374No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23900.10740361424396619No Hit
GGCCTGGATCCCTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTG23880.10731373674250681No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC23820.10704410423812866No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG23810.10699916548739893No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23760.10677447173375047No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG23540.10578581921769722No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23520.10569594171623785No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT22900.10290973917099688No Hit
CCGTAATACTTGTAGGCGGGGGTGTCAGTGTAGGAATTCTCCCACAGGCC22550.10133688289545764No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22360.10048304663159346No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)