FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005109597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313439
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT39900.17247050819148463No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA33300.14394155194928415No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT32620.141002204942512RNA PCR Primer, Index 39 (95% over 23bp)
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC30430.13153577855305457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28320.12241515769380563No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA28160.12172354663338865No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT27440.11861129686151224No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG26750.11562872416346401No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGT24620.10642165192166295No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT24440.10564358947869384No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCGCGTATGCCGTCTT24050.10395778751892744TruSeq Adapter, Index 11 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)