FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005109630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741532
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGAGCACGCTCGGGGATTTCGGCTATTCATGGTGT80320.4612031246052326No Hit
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC73000.41917116653613024No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT67070.3851206868435378No Hit
GTAATATACAGCCCTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA55550.31897203152167175No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53310.3061097929868644No Hit
GTCCTGATCTATGCTGTATCTAGTTTGCAAAGTGGGGTCCCATCAAGGTT37810.21710769598261762No Hit
GATCTATGCTGTATCTAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG34040.195460089162875No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG32670.18759345220185447No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31300.17972681524083392No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31220.17926744957887653No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC29030.16669231458279263No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26780.15377265534024068No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26760.1536578139247513No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25070.14395371431590115No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT24700.1418291481293482No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24410.1401639476047526No Hit
ATGCTGTATCTAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT24180.13884327132662508No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23730.13625933947811467No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT22660.1301153237494344No Hit
GAGTTACAGTACTCCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCA22260.12781849543964738No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21320.12242094891164791No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT21070.12098543121803103No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATC20650.11857376149275464No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20160.11576014681326556No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA19680.11300395284152114No Hit
ATATTACTGTGCGAGAGCACGCTCGGGGATTTCGGCTATTCATGGTGTAT19670.11294653213377646No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA19570.11237232505632971No Hit
GTATCTAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATC19420.11151101444015958No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA19410.11145359373241491No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18910.10858255834518116No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18870.10835287551420245No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18200.10450568809530919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)