FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005109635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330902
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT49100.2106480667140875No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT33740.14475083036524058TruSeq Adapter, Index 10 (95% over 21bp)
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC33200.14243413064985141No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA31300.13428277979940811No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27570.11828039102459048No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCGCGTATGCCGT27250.1169075319339895No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA26590.11407601005962499No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT26560.11394730451988115No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG26170.11227413250321121No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT24080.10330764656772357No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)