FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005109725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1414224
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31270.22111065856611117No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT31240.22089852809738766No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG31140.22019142653497606No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA25910.18321001482084875No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC23300.16475466404190567No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT19810.1400768195137404No Hit
GTATATTACTGTGCGAGAGCACGCTCGGGGATTTCGGCTATTCATGGTGT19510.13795551482650556No Hit
GTCCTGATCTATGCTGTATCTAGTTTGCAAAGTGGGGTCCCATCAAGGTT18950.13399574607700054No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA18160.1284096437339488No Hit
GATCTATGCTGTATCTAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG17950.1269247304528844No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG16850.11914661326635667No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG16490.11660104764167487No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16310.11532826482933396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.11419690232947538No Hit
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC14890.10528742264308906No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC14840.10493387186188327No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT14710.10401463983074817No Hit
GTAATATACAGCCCTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA14630.10344895858081889No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT14320.10125694373734287No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC14220.10054984217493126No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14190.10033771170620778No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)