FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005109779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129410
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT47770.22433444005616582No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG35110.16488135211161778No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT35050.1645995839223071Illumina PCR Primer Index 10 (95% over 21bp)
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA34920.1639890861788007No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC32980.15487858139108954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30950.14534542431941241No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA29110.13670453318055237No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT26660.12519899878370064No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT25710.12073766911961528No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT25100.11787302586162365No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT23820.11186197115632969Illumina PCR Primer Index 10 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)