FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005109783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370278
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT56210.23714517875118446No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT40230.16972692654616883Illumina PCR Primer Index 10 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38650.16306104178497205No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGT28580.12057657371835709No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT26610.11226531233889021Illumina PCR Primer Index 10 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGCGTATGCCGTCTT25440.10732918248407992No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC24490.10332121379855021No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)