FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005109799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339916
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52920.2261619647884796No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT47540.2031696864331882No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT41090.1756045943529597TruSeq Adapter, Index 11 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGTCTT28210.12055988334623978Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT27630.11808116188786265RNA PCR Primer, Index 11 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCGCGTATGCCGT27400.11709822061988549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23770.1015848432165941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)