FastQCFastQC Report
Sat 6 Aug 2022
EGAF00005109843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005109843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874074
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT34750.18542490851481852No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT29250.15607708126786882TruSeq Adapter, Index 12 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.15159486765197105No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGT21140.1128023760000939No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT20540.10960079484588121RNA PCR Primer, Index 12 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCGCGTATGCCGTCTT19040.10159684196034949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)