FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262881
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA52330.1603797380290608No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT52270.16019585145765353No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC50550.15492443641064446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44340.13589217626998962No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT42830.13126436422290608No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT40550.12427667450942895No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA40260.12338788941429368No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT39820.1220393878906402RNA PCR Primer, Index 41 (95% over 22bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG34800.10665421141623001No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCGCGTATGCCGT33960.10407979941652792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)