FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005110110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28877411
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT23173678.024843362862413No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT14206114.919454171289801No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG13900804.813727934266683No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC9916063.4338466145735853No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG6317232.1876026213014734RNA PCR Primer, Index 7 (97% over 36bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA4686231.6228012961411256No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC4061481.4064557241644688No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC4043341.4001739975927896No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA3791551.3129812779961472No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3423421.1855010132314148RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA3322061.15040091371072RNA PCR Primer, Index 1 (100% over 28bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG3117791.0796639629501412No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG2938711.0176500933549757No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAAC2855100.9886966667475834No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2656440.9199024109190398RNA PCR Primer, Index 2 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2401430.8315946329122095RNA PCR Primer, Index 1 (100% over 32bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC2338680.8098648455708166No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC2190810.7586587315601111RNA PCR Primer, Index 16 (100% over 51bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2014190.6974967388870145RNA PCR Primer, Index 1 (100% over 29bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC1977590.6848224724855009No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAAG1853350.6417992250067016No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGCC1612710.5584676548739083No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA1555090.5385143425773177No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1123800.3891623109841807RNA PCR Primer, Index 2 (100% over 34bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG1090300.37756154802104663No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG1029190.35639967862769967No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC998660.3458274012168196No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT996300.3450101534379242No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT982660.3402867383090541No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA928280.32145541025128604RNA PCR Primer, Index 1 (100% over 28bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA908460.3145919140742915No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC890970.30853527693324034No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGATA840460.29104409671628806No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC813970.28187083668961876RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC801840.27767032162266897No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACG794550.27514585708531836No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT782790.2710734698481107No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG771790.26726426409902193RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAGGA767260.26569556391326077No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAA742310.25705559269146394No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG734030.25418829963669526RNA PCR Primer, Index 1 (100% over 29bp)
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAAT719870.2492848129633228No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGGA710000.24586691653209494RNA PCR Primer, Index 1 (100% over 22bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC698810.2419919154109764No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT689580.2387956454960592RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG672970.23304374481493512RNA PCR Primer, Index 1 (100% over 29bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA671470.23252430766733206No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA666160.23068550016481743No Hit
TGCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAA660480.22871856483256064No Hit
ATTCGTGATGACTGATCATTTCTTCACTTTGACCAGATGTCTACTGAAGAA658460.22801905614045526No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC653620.22634300561085616RNA PCR Primer, Index 1 (100% over 33bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA633410.21934445577548484No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG627800.2174017608434496RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT627340.21724246678485132No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA597270.2068294834325695No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC586600.20313455385595336No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGGAATTCTCGGGTGCC572700.19832110295483207No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC571270.19782590620745052RNA PCR Primer, Index 5 (97% over 35bp)
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC566650.1962260397928332RNA PCR Primer, Index 5 (97% over 35bp)
GGGCGGTGATGACCCCAACATGCCATCTGAGTGTCGGTGCTGAAATCCAGA565460.19581395298906815No Hit
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG562760.19487896612338274RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA551800.19108361203156335No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG544770.18864918326646388No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTC537480.1861247187291132No Hit
GATCGATGATGAGCACTGGTGGAGTATGAGTCACATACGATGAATACGTGT520630.18028970810437264No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCC509840.17655322355594827No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGAT508860.17621385795284764No Hit
ACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTCCA508560.17610997052332703RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC507340.1756874949766099RNA PCR Primer, Index 16 (100% over 44bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA506840.17551434926074225RNA PCR Primer, Index 1 (100% over 28bp)
GTTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA500670.17337773112693516No Hit
AGCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC493720.17097100567637452No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTCT490160.1697382081793967No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA488960.16932265846131428RNA PCR Primer, Index 1 (100% over 22bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT477540.16536801031089662No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGGTGCCAAGGAACTCC477260.16527104871001075RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG474440.16429450687251707No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC464220.16075540844018185RNA PCR Primer, Index 1 (100% over 27bp)
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATGA464020.16068615015383478No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG461930.15996240106150791RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA461730.15989314277516084No Hit
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG458290.15870190024999126No Hit
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC457280.15835214590393856RNA PCR Primer, Index 1 (100% over 27bp)
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGATT456220.1579850769862991No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA449000.15548485284916988RNA PCR Primer, Index 1 (100% over 28bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACAT436290.15108348875181365No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC429070.14858326461468446No Hit
GTCCAGTTTTCCCAGGAATCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA425400.14731237506021574RNA PCR Primer, Index 1 (100% over 28bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATAACTATGAAAGAAAAC421130.14583371064670583No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA420690.14568134241674227RNA PCR Primer, Index 1 (100% over 22bp)
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTGGAATTCTCGGGTGC416260.1441472713741547No Hit
TGGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCG415110.14374903622765905No Hit
TCCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAGG413470.1431811182796131No Hit
GACCTATGATGATGACTGGTGGCGTATGAGTCATTGACGGTGAATACAGGT410020.14198641284012614No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG402600.13941693041664988No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC401670.139094879385136No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATGA395040.13679896719273069No Hit
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA388540.13454807288645093RNA PCR Primer, Index 1 (100% over 28bp)
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAACT388410.13450305500032533No Hit
TGCCTCTGATGAAGCCTGTGTTGGTAGGGACATCTGAGAGTAATGATGAAT377230.1306315167935242No Hit
TTCAGATGATGAATTTAACTGTTCAACTGCTGAATGATAACGGGCATGAAC370720.12837715957292709No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG370430.12827673505772386Illumina Small RNA Adapter 2 (100% over 21bp)
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTAA370260.12821786551432882No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGGG367830.12737637733521193No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTTT364850.1263444288686406No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG355100.122968087409221No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGC354510.12276377546449714No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC344830.11941167440529901No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG337340.11681795158160128No Hit
ATCGATGATGACTACCGGTGGCGTATGAGTCATATGTGATGAATACGTGTT332550.11515921562358898No Hit
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT331480.11478868379163215No Hit
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG323350.1119733344516238RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC310940.10767585778378816RNA PCR Primer, Index 1 (100% over 27bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAA306300.10606906554053618RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCCA303630.1051444674178028No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG301380.10436531169639826RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG301250.10432029381027268No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG300500.10406057523647115No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG299010.10354460100318551RNA PCR Primer, Index 1 (100% over 29bp)
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATACT297150.10290049894015776No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAGCGCCCGAATCGGAC293210.1015361106991205No Hit
CGAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA292950.10144607492686931RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)