FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54362811
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT54052219.942865169352629RNA PCR Primer, Index 8 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC18264673.3597729153483256RNA PCR Primer, Index 25 (100% over 51bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA16715913.074879626809585RNA PCR Primer, Index 1 (100% over 34bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG16102842.962105841068446No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG14443502.656871441029788No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA11815892.173524470616503RNA PCR Primer, Index 1 (100% over 32bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC11087312.0395027034198066RNA PCR Primer, Index 5 (100% over 35bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT9110631.6758938385286957No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT8436291.5518494803368428No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC7771531.429567356257571No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT7264461.3362921943090837No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC6228021.1456398014444102No Hit
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC6154781.1321673560993748RNA PCR Primer, Index 5 (100% over 35bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG5675291.043965515322598RNA PCR Primer, Index 25 (100% over 45bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG5652351.0397457188150185No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG5554421.0217315657205437RNA PCR Primer, Index 5 (97% over 37bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC5458141.0040209289398225RNA PCR Primer, Index 1 (100% over 33bp)
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA4663250.8578014849158554No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC4367180.803339621271608RNA PCR Primer, Index 5 (100% over 35bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3944160.7255253963964446RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA3614970.6649711325633989RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT3224570.5931573332365024RNA PCR Primer, Index 8 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC2888690.5313724487131469No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC2679000.49280012396709955No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA2609750.48006163625350423No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT2264540.4165605049378333No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC2250890.4140495972513268RNA PCR Primer, Index 5 (100% over 35bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2213780.4072232394310883RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA2168370.3988701025780289No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA2139960.3936441035030363No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAAC2085110.38355448543674464No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAAG2074870.3816708447986621No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA2071280.38101046687964685RNA PCR Primer, Index 25 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG1823370.3354076006113812No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG1820590.33489622161002675No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1803470.3317470099182325RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG1787310.32877438953625854RNA PCR Primer, Index 1 (100% over 29bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1782920.3279668521923931RNA PCR Primer, Index 1 (100% over 31bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCA1723450.31702738844759154No Hit
GCGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1690220.31091475383787637RNA PCR Primer, Index 1 (100% over 32bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC1678160.3086963255082597No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1645710.30272717133777355RNA PCR Primer, Index 1 (100% over 33bp)
TTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGA1636780.30108450425788325No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1620230.2980401436562947RNA PCR Primer, Index 1 (100% over 29bp)
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAA1549500.28502941100672663No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1522150.2799983981696605RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC1512200.2781681028230862RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1508370.27746357707661584RNA PCR Primer, Index 1 (100% over 33bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1494020.27482390489336544RNA PCR Primer, Index 1 (100% over 34bp)
GGGCGGTGATGACCCCAACATGCCATCTGAGTGTCGGTGCTGAAATCCAGA1416920.26064141532342766No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC1303200.23972270308097202No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1258140.23143394847628465No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG1234640.22711114037131008No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1191920.21925282708430954RNA PCR Primer, Index 1 (100% over 34bp)
CACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1120100.20604158971838302No Hit
CCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1117490.2055614820948093RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1110670.2043069479979613RNA PCR Primer, Index 1 (100% over 29bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA1065800.19605314375667587RNA PCR Primer, Index 25 (100% over 40bp)
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGATA1055080.1940812074636832No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1027900.18908146600439776RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC1020990.18781037647225418No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1019790.18758963733497888RNA PCR Primer, Index 1 (100% over 29bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC1009090.18562138002760747No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA965900.17767661057850742RNA PCR Primer, Index 1 (100% over 22bp)
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC921030.16942280633722195No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC910880.1675557211344351No Hit
CGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC900490.16564448810419313RNA PCR Primer, Index 1 (100% over 31bp)
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT898250.1652324417146126No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGGA880710.162005971324772RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG880290.16192871262672565No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG877950.16149827130903882RNA PCR Primer, Index 1 (100% over 29bp)
GTCCAGTTTTCCCAGGAATCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA841590.15480987544959735RNA PCR Primer, Index 1 (100% over 28bp)
TAATGTGAATTGCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGT840430.15459649428356453RNA PCR Primer, Index 1 (100% over 30bp)
TAATGTGAATTGCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA830270.15272756958796704RNA PCR Primer, Index 1 (100% over 32bp)
TGCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAA815530.15001615718510214No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGGAATTCTCGGGTGCC795980.14641994874032546No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG791520.1455995349467856RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC789170.14516725413628814RNA PCR Primer, Index 1 (100% over 27bp)
AATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG786510.14467794904866124No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGA764900.14070280508489527No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC738350.13581895167267932RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA731830.13461960236015022No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC726690.13367410305548771No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA714160.13136921856377148RNA PCR Primer, Index 1 (100% over 28bp)
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAAT707680.13017722722248487No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATGA703130.12934025799364937No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG690830.12707768183657758RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA680860.125243707504382No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA670350.12331040056041254RNA PCR Primer, Index 1 (100% over 28bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTG669090.12307862446627345No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA663710.12208897733415587RNA PCR Primer, Index 1 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAAG639140.1175693434984442No Hit
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT618710.11381125968633225RNA PCR Primer, Index 25 (100% over 39bp)
TGTCTGAGCGTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT600330.11043027190039897RNA PCR Primer, Index 8 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCGG591630.10882991315515307No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTGAACTTTCAAGTGAT589190.10838107690935997No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC577110.10615896959412198RNA PCR Primer, Index 1 (100% over 27bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAA569690.10479406592863641RNA PCR Primer, Index 1 (100% over 23bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC561470.10328200283830061RNA PCR Primer, Index 5 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTC556830.10242847817416947No Hit
AACTGGCCCTCAAAGTCCCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG555040.10209920896106717RNA PCR Primer, Index 1 (100% over 29bp)
CTGCGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT546400.10050988717268503RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGA411650.044.918451
GTCTACG195750.044.569961
ACCCGTA536350.044.5074352
CCCTTTA261850.044.50723310
TTTCTAT1513400.044.480451
CTACATC102750.044.4803923
ATCCGTC147400.044.45585
GCTACAT121100.044.449242
AGCTACA121300.044.4191441
CCTAACT288200.044.40348411
AGTTTTC114900.044.35915
CCCGTAG538450.044.3331573
CACAGAT652800.044.3215561
TTGTCCC276200.044.321429
ACTCGTC186400.044.315037
TTAACCT263050.044.3038314
ATTGCAC465250.044.29572
CCGTAGA539450.044.2716674
GACCCTA289300.044.2666938
TACATCT103350.044.2224
Produced by FastQC (version 0.11.5)