FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42453401
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG28765756.775841115768322RNA PCR Primer, Index 15 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC27891846.56998952804747RNA PCR Primer, Index 26 (100% over 51bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC20228924.764970420155501RNA PCR Primer, Index 1 (100% over 33bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG17278044.069883588360801No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT16543913.896957513486375RNA PCR Primer, Index 1 (100% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA10644062.5072337549587607RNA PCR Primer, Index 1 (97% over 37bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC9018152.124246771183303No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA8745802.060094078210601RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG6393881.5060937049542862RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA6170051.4533700138653203RNA PCR Primer, Index 1 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG5622681.3244357030429672RNA PCR Primer, Index 15 (100% over 36bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT5270351.2414435300483937RNA PCR Primer, Index 1 (100% over 35bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT5096531.2004998139018355No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA4510521.0624637587928467RNA PCR Primer, Index 1 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4456471.049732152201422RNA PCR Primer, Index 1 (100% over 32bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG3774790.8891608000970287RNA PCR Primer, Index 1 (100% over 29bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT3728610.8782829908020796No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC3370250.7938704369056322No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2809590.6618056348418352RNA PCR Primer, Index 1 (100% over 34bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2753200.6485228356616234RNA PCR Primer, Index 1 (100% over 29bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA2722030.6411806677161154No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT2505850.5902589523981836RNA PCR Primer, Index 1 (100% over 35bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2383120.5613496077734738RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA2221680.5233220301949425No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC2201540.5185780050931609No Hit
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1934530.45568316187435726RNA PCR Primer, Index 1 (100% over 35bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1909910.4498838620726759RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1604640.3779767844748175RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1589620.37443878760149274RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG1508360.355297800522507RNA PCR Primer, Index 26 (100% over 38bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC1461770.34432341474832606RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC1455080.3427475692701275No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1427120.33616152449128867RNA PCR Primer, Index 1 (100% over 29bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1396940.3290525534102674RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1330930.3135037402539316RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1275760.3005083149875319RNA PCR Primer, Index 1 (100% over 30bp)
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAAG1196540.2818478547808219No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1183080.2786773196333552RNA PCR Primer, Index 1 (100% over 33bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1173310.2763759727989755RNA PCR Primer, Index 1 (100% over 29bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC1140830.2687252312247021No Hit
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1130650.26632730791109055RNA PCR Primer, Index 1 (100% over 32bp)
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAAC1107230.26081067097545374No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGGA993860.23410609670589172RNA PCR Primer, Index 1 (100% over 22bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG991580.233569037260407No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCG978870.2305751664042181RNA PCR Primer, Index 26 (100% over 45bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAT911280.21465418047425694RNA PCR Primer, Index 26 (100% over 41bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC904380.21302886899450058No Hit
TCCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC898480.2116391099031147RNA PCR Primer, Index 1 (100% over 33bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT898430.21162733228369618No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG835530.1968110870551926No Hit
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG804480.189497185396289RNA PCR Primer, Index 1 (100% over 29bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA797690.1878977846792534No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC783150.1844728529523465RNA PCR Primer, Index 1 (100% over 27bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC743300.17508609027578262RNA PCR Primer, Index 26 (100% over 39bp)
GCTTTGCACGTATGAGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC742110.17480578293362176RNA PCR Primer, Index 1 (100% over 26bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG741240.1746008523557394RNA PCR Primer, Index 1 (100% over 29bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG738380.1739271725249998No Hit
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC726880.17121832005873924RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG716470.16876621969580247No Hit
AACTGGCCCTCAAAGTCCCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG683250.16094116935413488RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAACTCGTATGCC665590.1567813141755121RNA PCR Primer, Index 26 (98% over 51bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC653000.1538157096059277No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA651640.15349535835774383RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGGCTGTGTGCACATGTGCTGGAATTCTCGGGTGCCAAGGAACTCCA650900.15332104959034965RNA PCR Primer, Index 1 (100% over 28bp)
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT643370.1515473401059199No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCA629550.14829200609863977RNA PCR Primer, Index 26 (100% over 40bp)
CCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA624630.147133088347857RNA PCR Primer, Index 1 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCA623950.14697291272376506RNA PCR Primer, Index 26 (100% over 40bp)
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC593780.13986629716662746No Hit
TAATGTGAATTGCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA589470.13885106637275066RNA PCR Primer, Index 1 (100% over 32bp)
TAATGTGAATTGCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGT580740.13679469402227631RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG561460.13225324397449337RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCCA560680.13206951311156437RNA PCR Primer, Index 1 (100% over 28bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA554560.130627932494737RNA PCR Primer, Index 1 (100% over 22bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC548090.12910390854197995RNA PCR Primer, Index 1 (100% over 33bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG540800.1273867316307591RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT539000.12696273733169222No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT535150.12605586063646584RNA PCR Primer, Index 1 (100% over 30bp)
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATGA513190.12088313018784996No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC508350.11974305662813681No Hit
GCGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA502390.11833916439344871RNA PCR Primer, Index 1 (100% over 32bp)
GAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG501040.11802116866914857RNA PCR Primer, Index 1 (100% over 29bp)
GTACTTGGATGGGAGACCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT499670.117698461897081RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAGCTCGTATGCC491380.11574573259748966RNA PCR Primer, Index 26 (98% over 51bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA488840.11514742953102862RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC480520.11318763365978618RNA PCR Primer, Index 1 (100% over 33bp)
CACGCTCATGCACACACCCACATGGAATTCTCGGGTGCCAAGGAACTCCAG477830.11255399773506956RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC475860.11208995952997972No Hit
TCCCCGGCATCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC465640.10968261412083334RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTC463180.10910315524544194RNA PCR Primer, Index 26 (100% over 44bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA462830.10902071190951226RNA PCR Primer, Index 1 (100% over 22bp)
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT461790.10877573742560696RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA457410.10774401796454422No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTCT452710.10663692173920294No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC452030.10647674611511103RNA PCR Primer, Index 1 (100% over 27bp)
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATGA451340.10631421496713539No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCA437810.1031271911524827RNA PCR Primer, Index 26 (100% over 40bp)
AACCCGTAGATCCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC426230.10039949449515247RNA PCR Primer, Index 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGA966900.045.036121
TTTCTAT1792650.044.8132631
AGCTACA120650.044.7323951
CCCTTTA256500.044.72517410
AACCCGT569450.044.7231981
GTCTACG70650.044.7045021
GCTTATC84600.044.6333353
ATCCGTC437950.044.624765
ACCCGTA1507600.044.5993422
CACCCGT937150.044.594271
AAGCTGC147850.044.5842361
TTCCGTA352300.044.5586053
CCCGTAG1509100.044.5509223
GTAGGTT94300.044.5441938
TCCGTCT439800.044.543926
AGTAGGT95300.044.5309527
TATTGCA625750.044.5291
CGTAGAT571700.044.524785
CTCGTCC258800.044.5193638
CACAGAT949050.044.5030631
Produced by FastQC (version 0.11.5)