FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73790557
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC53642977.269625299074514No Hit
TTAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC23469723.1805858302438343RNA PCR Primer, Index 1 (100% over 33bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG16589112.248134541117504No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC15586482.1122594317861023RNA PCR Primer, Index 42 (100% over 51bp)
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC14192801.9233897367111623No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG11155651.5117991316964852RNA PCR Primer, Index 1 (100% over 29bp)
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGC10995511.4900971678530628No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA7402281.003147326832077No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG6370660.8633435305278967RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA6301510.8539724127573667RNA PCR Primer, Index 3 (97% over 36bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCG6191710.8390924600284559RNA PCR Primer, Index 42 (100% over 39bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA5817510.7883813642984155RNA PCR Primer, Index 7 (97% over 35bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA5622200.7619132079461062No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA5592070.7578300296608412RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA5562230.7537861517971738No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC5180360.7020356276752322No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGAT4847900.6569810822813006RNA PCR Primer, Index 42 (100% over 41bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT4578560.6204804769260652No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT4509710.6111500147640843No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA4304450.5833334473949018RNA PCR Primer, Index 42 (100% over 40bp)
CGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA4161300.5639339461823007RNA PCR Primer, Index 3 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA3989680.5406762277184057No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT3541140.47989067218993886No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC3062360.4150070313197392RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT3056920.41426980961805177RNA PCR Primer, Index 3 (100% over 34bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC3003180.4069870349399856No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC2994670.4058337708441474No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA2836620.38441504107361596RNA PCR Primer, Index 42 (100% over 40bp)
CCCGCGAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGTGGAATTCTCGGGTG2833720.3840220368576429No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2786520.37762555444594353RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT2758320.37380392724234346RNA PCR Primer, Index 42 (100% over 37bp)
TAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2612380.35402632887023744RNA PCR Primer, Index 3 (100% over 34bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2524680.34214133930443164RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2365210.3205301729867685RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA2233360.3026620330295108RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2193440.29725212671859896RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA2131510.2888594539271468No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA2100160.2846109428337829No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT2088510.28303215003513255No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2073680.28102240778586346RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2034490.27571143012242066RNA PCR Primer, Index 1 (100% over 31bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1917310.259831349423206RNA PCR Primer, Index 3 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTCT1915080.2595291427329922No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1868320.25319228854716463Illumina Small RNA Adapter 2 (100% over 21bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA1811360.2454731436706732RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1715060.23242269332646454RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGGA1654230.2241790910996918RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGATCGGCCCCGCCGGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCA1565740.2121870417647071RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATC1562460.21174254044457205RNA PCR Primer, Index 42 (100% over 42bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA1554570.21067329793973502No Hit
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1478440.20035625967696652RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1476990.20015975756898RNA PCR Primer, Index 1 (100% over 33bp)
CCCTGCGGGCCGCCGGTGAAATACCACTACTCTGTGGAATTCTCGGGTGCC1409400.19100004896290457No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1405700.190498629790801RNA PCR Primer, Index 1 (100% over 29bp)
GGCGGATGTGGCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1398130.18947275326841617RNA PCR Primer, Index 7 (97% over 35bp)
TCCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1382270.18732342676312905RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG1379110.1868951876864136No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1361410.1844965067820263RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA1356330.18380807181059766RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCCA1339320.18150289880587295RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA1333270.18068301070013607RNA PCR Primer, Index 3 (97% over 36bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC1322530.17922753991408413RNA PCR Primer, Index 1 (100% over 24bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC1302190.1764710896544662No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1300820.17628542904209274RNA PCR Primer, Index 1 (100% over 29bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1261870.17100697586548913RNA PCR Primer, Index 1 (100% over 32bp)
CTAGAGGTGAAATTCTTGGACCGGCGCAAGACGTGGAATTCTCGGGTGCCA1252750.16977104536560145No Hit
GTCCAGTTTTCCCAGGAATCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA1240540.16811636209765973RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG1239240.16794018779394768Illumina Small RNA Adapter 2 (100% over 21bp)
AATTGCAGGACACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGG1228160.1664386406515403No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1141020.1546295415550258RNA PCR Primer, Index 1 (100% over 33bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1138180.15424466846076254RNA PCR Primer, Index 3 (100% over 34bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1123290.15222679508978365RNA PCR Primer, Index 1 (100% over 29bp)
ACACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGGTGCCAAGGAAC1102790.14944866183893962RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1101960.14933618132195425RNA PCR Primer, Index 1 (100% over 29bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1100150.149090892483709RNA PCR Primer, Index 7 (97% over 35bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1085070.1470472705606491No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1078380.14614065049000782RNA PCR Primer, Index 1 (100% over 29bp)
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACG1073640.14549829187493463No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG1059950.14364304093815147No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAT1057490.1433096649480502RNA PCR Primer, Index 42 (100% over 37bp)
ACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAA1051050.1424369245511997RNA PCR Primer, Index 1 (100% over 23bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1040460.14100178156942222RNA PCR Primer, Index 3 (100% over 34bp)
CACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAAC1019300.13813420597977055RNA PCR Primer, Index 1 (100% over 24bp)
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGAT1000080.13552953665873535RNA PCR Primer, Index 42 (100% over 41bp)
CACTTCGAACGCACTTGCGGCCCCGGTGGAATTCTCGGGTGCCAAGGAACT983720.13331245080586668RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGAGACCCTTTAACCTGTGGTGGAATTCTCGGGTGCCAAGGAACTCC983590.13329483337549544RNA PCR Primer, Index 1 (100% over 27bp)
AATTGCAGGACACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGT977060.13240989629608027No Hit
CCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA972860.13184071777639514RNA PCR Primer, Index 7 (97% over 35bp)
ACGAGGCGCCGCCGCCCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT968540.13125527701329048RNA PCR Primer, Index 1 (100% over 30bp)
TGAATTGCAGGACACATTGATCATCGACACTTCGAACGCACTTGCGGCCCC943930.12792016192532601No Hit
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCCA915670.12409040360001619RNA PCR Primer, Index 1 (100% over 28bp)
GGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCC905230.1226755884225132No Hit
CCGAGGGGCTCTCGCTTCTGGCGCCTGGAATTCTCGGGTGCCAAGGAACTC895920.12141390937054453RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC887600.12028639382678735RNA PCR Primer, Index 42 (100% over 38bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT880340.11930252809990308RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCGG872650.11826038933409866No Hit
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATGA863310.1169946447212751No Hit
CGCGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT838140.11358363916401933RNA PCR Primer, Index 3 (100% over 34bp)
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA832840.11286539007965476RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCG826540.11201162230012711RNA PCR Primer, Index 42 (100% over 39bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA823570.11160913177549264RNA PCR Primer, Index 7 (97% over 35bp)
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC820990.11125949354197177RNA PCR Primer, Index 42 (100% over 38bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG817040.11072419469607744No Hit
ACACTTCGAACGCACTTGCGGCCCCGTGGAATTCTCGGGTGCCAAGGAACT809310.1096766351824665RNA PCR Primer, Index 1 (100% over 25bp)
GCGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA787510.10672232762791044RNA PCR Primer, Index 7 (97% over 35bp)
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC785580.10646077654624563RNA PCR Primer, Index 42 (100% over 38bp)
CCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT783450.10617212172554817RNA PCR Primer, Index 3 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA782600.10605693083465952RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT764400.10359049058269068No Hit
CACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA759010.10286004481576144RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTCT740720.10038140788122794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG808050.044.9353141
TTTCTAT1720350.044.7201841
CCCTTTA867100.044.70729410
TTAACCT868750.044.666814
ACCCGTA2932400.044.6348232
TCCCTGA1306800.044.596241
CGTAGAT1215250.044.58725
CCCGTAG2936300.044.5812233
CCGTAGA2937750.044.554664
GACCCTT870650.044.5223588
AACCCGT1219750.044.4928781
ATCCGTC235550.044.4651645
TAGTAGG193900.044.4546366
CACCCGT1726350.044.4525831
ACCTTGC1709050.044.4034615
CCTTGCG1454950.044.3850216
TTAAGTA2543950.044.378851
AACCGAC1728950.044.37460310
CAGGACG1492100.044.364071
CTACGGC818750.044.3635753
Produced by FastQC (version 0.11.5)