FastQCFastQC Report
Wed 8 May 2024
EGAF00005110114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33680434
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA14210284.2191499076288626RNA PCR Primer, Index 9 (100% over 35bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA12095043.591117620396459RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCA10842833.219326093006996RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGC7349362.182085895924025RNA PCR Primer, Index 41 (100% over 50bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA6891792.0462295705571965No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC6859282.0365770821124216RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG6404531.9015580381179171RNA PCR Primer, Index 6 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC5242391.556509040233864No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT4833991.4352516953908612No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG3971411.179144544277547No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC3849131.1428385988137801RNA PCR Primer, Index 41 (100% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3640211.0808085192726435RNA PCR Primer, Index 1 (100% over 33bp)
TTAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3598801.06851354706415RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA2939640.8728034799076521RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTC2696800.8007022712355785RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2629110.7806045492169132RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA2421260.718892161543999RNA PCR Primer, Index 1 (100% over 28bp)
GGCGGATGTGGCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2244290.6663483018063246RNA PCR Primer, Index 6 (100% over 34bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG2141870.6359389549433954RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1979690.5877863687861029RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1965550.5835880855929588RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1945330.5775846000084203No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC1905280.565693423071686RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1721470.511118710643693No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC1703920.5059079701882702No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1645700.48862196965751686RNA PCR Primer, Index 1 (100% over 29bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1613470.4790526155333984RNA PCR Primer, Index 1 (100% over 32bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC1552440.4609323027132014No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACT1549790.4601454957498469No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA1447750.4298489740363797RNA PCR Primer, Index 9 (100% over 35bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGT1371910.4073314494700395No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1346430.39976622629031444RNA PCR Primer, Index 6 (100% over 34bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1326740.39392010209844686RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1319940.3919011257396505No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTC1301280.38636081708448294RNA PCR Primer, Index 41 (100% over 44bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCC1299840.385933269149679RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCC1161670.344909450988666RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1159330.3442146855946096RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1107140.32871904204084784No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGA1084260.32192578041007425RNA PCR Primer, Index 41 (100% over 38bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCC1076100.3195030087795187RNA PCR Primer, Index 1 (100% over 27bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC1065870.31646563699268243No Hit
CCAGTGCGCCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1053110.3126770872370588RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA951570.2825290196676207RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA927840.2754833859919976RNA PCR Primer, Index 1 (100% over 28bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCG864840.2567781638443258No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA859760.2552698697409897RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC818040.2428828559631981RNA PCR Primer, Index 1 (100% over 26bp)
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGAC805730.23922791493720064RNA PCR Primer, Index 41 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT803380.23853018046026364No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC795310.23613413057563334RNA PCR Primer, Index 1 (100% over 31bp)
AACCCGTAGATCCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT792750.23537404535820414RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT719060.2134948736111892RNA PCR Primer, Index 1 (100% over 30bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG716980.212877304372028RNA PCR Primer, Index 1 (100% over 29bp)
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA712630.21158575331897445RNA PCR Primer, Index 1 (100% over 32bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA694660.20625031138256708RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG680880.20215891517312395RNA PCR Primer, Index 6 (100% over 34bp)
CGGCCGGGCGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC675570.20058233216353447RNA PCR Primer, Index 41 (100% over 36bp)
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTCC675020.2004190326051024RNA PCR Primer, Index 1 (100% over 27bp)
CAGTGCGCCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA668790.1985692939704993RNA PCR Primer, Index 9 (100% over 35bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG668020.19834067458869442RNA PCR Primer, Index 6 (100% over 34bp)
GTCCAGTTTTCCCAGGAATCCCTTGGAATTCTCGGGTGCCAAGGAACTCC667240.19810908612400896RNA PCR Primer, Index 1 (100% over 27bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG657150.1951132814975009No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGG650120.19302601623245116No Hit
CACCCGTAGAACCGACCTTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC644910.1914791240516675RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC635520.18869115522680022RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGAGACCCTTTAACCTGTGGTGGAATTCTCGGGTGCCAAGGAACTC618380.18360214716948126RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG617790.18342697127952687Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC598460.17768773407136024RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG595580.17683263820175238No Hit
GCTTTGCACGTATGAGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGAACT581280.17258684968251894RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC578710.17182379538220915RNA PCR Primer, Index 1 (100% over 33bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA568770.1688725269989098RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG564890.16772052284124367RNA PCR Primer, Index 1 (100% over 29bp)
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACAT541730.16084412688981384RNA PCR Primer, Index 41 (100% over 41bp)
TCCCTGAGACCCTAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA536140.15918440955956803RNA PCR Primer, Index 1 (100% over 28bp)
TAATGTGAATTGCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTC527710.1566814726912367RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT517770.15373020430793735No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC513110.15234661168558578No Hit
CCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCAAGGAACT504370.14975163324795635RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG500050.14846898944354459No Hit
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT487180.14464777977623447RNA PCR Primer, Index 1 (100% over 30bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACG482370.14321965091067412RNA PCR Primer, Index 41 (100% over 37bp)
CCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG480670.14271490682097504RNA PCR Primer, Index 6 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA479540.14237940045546918No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC478380.1420349868413216No Hit
CGGCGCGCGGCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC465430.13819002451096682RNA PCR Primer, Index 1 (100% over 33bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACA460840.1368272154687793RNA PCR Primer, Index 41 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA453380.1346122796398645No Hit
TCCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA451070.13392642149444986RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCT443380.13164319675928168RNA PCR Primer, Index 41 (100% over 43bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA438870.13030414038013882RNA PCR Primer, Index 1 (100% over 28bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGAC436720.12966578756081348RNA PCR Primer, Index 41 (100% over 39bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACA425210.12624837316526266RNA PCR Primer, Index 41 (100% over 40bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCGTGGAATTCTCGGGTGCC416250.12358807490426044No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG415340.1233178883621274No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA414570.12308926898032253No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGT403590.11982921597744257RNA PCR Primer, Index 1 (100% over 30bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA400140.11880488238364151RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGAGACCCTAACTTGTGAAATGGAATTCTCGGGTGCCAAGGAACTC391510.1162425638576985RNA PCR Primer, Index 1 (100% over 26bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG390890.11605848071910238Illumina Small RNA Adapter 2 (100% over 21bp)
TAATGTGAATTGCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAG384530.1141701440070517RNA PCR Primer, Index 1 (100% over 29bp)
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGGTGCCAAGGAACTC382000.11341896603826424RNA PCR Primer, Index 1 (100% over 26bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA374020.11104963789955913RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA367680.10916723935326961RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGAC364750.10829729806925885RNA PCR Primer, Index 41 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA347790.10326173350379036No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA343660.10203550227410965No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)