FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51972398
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC529088410.180180641270391RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC21305664.09941831046549No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC16749263.2227221841870755RNA PCR Primer, Index 1 (100% over 33bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA13623152.6212279063975457RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA12353172.3768712769420413No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA8032231.5454799680399585No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA6651081.2797331383477824RNA PCR Primer, Index 2 (97% over 36bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG6019191.158151294077291Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTCTCGGGTGCCAAGGA5319581.023539456463025RNA PCR Primer, Index 1 (100% over 22bp)
TAAGTAAGAGTATTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC4289450.8253323235152629RNA PCR Primer, Index 2 (100% over 34bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG3872380.7450839578346953RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3766540.7247193019648622RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG3702520.7124012249733022No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAATCTCGTATGCC3672710.7066654880923524RNA PCR Primer, Index 29 (100% over 51bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG3590150.690780132946723RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCA3350670.6447018280742021No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA2797770.5383184358743655RNA PCR Primer, Index 7 (100% over 35bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG2439080.46930295577279313No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2434110.4683466789429266RNA PCR Primer, Index 1 (100% over 32bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT2410990.4638981637907106No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC2384390.45878006244776315RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2325830.4475125431002818RNA PCR Primer, Index 1 (100% over 32bp)
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC2325140.447379780321085No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG2243660.4317022277863723Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGC2237310.4304804253981123No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGGA1979490.38087332433650645RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1878320.36140722234906303RNA PCR Primer, Index 1 (100% over 29bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1858670.35762636928932934RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA1828130.3517501732361859No Hit
GGCGGATGTGGCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1780820.34264726441908644RNA PCR Primer, Index 7 (100% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1748290.3363881728143466RNA PCR Primer, Index 2 (100% over 34bp)
TCCCTGAGACCCTAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG1683110.32384690042587605RNA PCR Primer, Index 1 (100% over 29bp)
CCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCCAAGGAACTC1660210.3194407154351431RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG1576040.30324558047138794Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1470290.2828982414857979RNA PCR Primer, Index 2 (100% over 34bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1435410.2761869867924894RNA PCR Primer, Index 2 (100% over 34bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA1395020.26841555396385597RNA PCR Primer, Index 1 (100% over 22bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1369420.26348986244583134RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC1364890.2626182459389309No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTA1359770.26163310763532593RNA PCR Primer, Index 29 (100% over 39bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1348900.2595416128384147RNA PCR Primer, Index 1 (100% over 32bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1317260.2534537659778562RNA PCR Primer, Index 1 (100% over 28bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC1255180.24150896404664646No Hit
TAATGTGAATTGCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1224950.23569241503922908RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1186800.22835198021842287RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1174170.22592184412964744RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTCT1121220.21573374389998323No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG1119260.21535662064313446No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA1115860.21470242723839683No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA1051210.20226313205713542RNA PCR Primer, Index 1 (100% over 28bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA1025440.1973047308688739RNA PCR Primer, Index 1 (100% over 28bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA1005040.193379570440448No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG966690.18600065365465723No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG963210.18533106746392575No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT959650.18464608848720046RNA PCR Primer, Index 1 (100% over 30bp)
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC945380.18190040028555157RNA PCR Primer, Index 1 (100% over 31bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTC913320.17573174129852542No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAAT863390.16612471874012819RNA PCR Primer, Index 29 (100% over 41bp)
AGCGGAGTAGAGCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA837670.1611759380431128RNA PCR Primer, Index 7 (100% over 35bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA825340.15880352490181424RNA PCR Primer, Index 7 (100% over 35bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT821840.1581300905145843RNA PCR Primer, Index 1 (100% over 30bp)
CACGGTCCCCCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCAAGGAA818260.15744126334136055RNA PCR Primer, Index 1 (100% over 23bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT793670.15270990574650797No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA791470.15228660413167774RNA PCR Primer, Index 1 (100% over 22bp)
CACCCGGCCCGGACACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC762950.14679907592487842RNA PCR Primer, Index 2 (100% over 34bp)
TAATGTGAATTGCAGGACACATGGAATTCTCGGGTGCCAAGGAACTCCAGT755110.1452905828974834RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG752320.14475375948594868RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA748460.14401105756174654RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCAA739470.14228129323569022No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC739180.1422254943864626No Hit
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA732230.14088824610324888RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCCA732150.14087285331725505RNA PCR Primer, Index 1 (100% over 28bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAGG726480.13978188960994256Illumina Small RNA Adapter 2 (100% over 21bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC718950.13833304362827362RNA PCR Primer, Index 1 (100% over 33bp)
CTAGAGGTGAAATTCTTGGACCGGCGCAAGACGTGGAATTCTCGGGTGCCA704920.13563353378460621No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC703470.1353545395384681RNA PCR Primer, Index 1 (100% over 27bp)
CAACCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGG702010.13507362119408076Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC684110.13162948532796198RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA682580.13133509829583004No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC678650.13057892768388327RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA660720.1271290195230168RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG654430.12591876172425218RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC651510.1253569250354775RNA PCR Primer, Index 1 (100% over 27bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC637360.12263432601281934No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG625270.12030809122950226No Hit
AACCCGTAGATCCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC612390.1178298526844961RNA PCR Primer, Index 1 (100% over 31bp)
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA608100.11700441453557714RNA PCR Primer, Index 29 (100% over 40bp)
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA593810.1142548781374298No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT590170.11355450637471068RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCGG584380.11244045348840744No Hit
CGAATGTTGCTCGGTGAACCCCTTGGAATTCTCGGGTGCCAAGGAACTCCA577380.11109358471394759RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC568500.10938498546863279RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG555110.106808617912916RNA PCR Primer, Index 1 (100% over 29bp)
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAACTAA551790.1061698172941722RNA PCR Primer, Index 29 (100% over 40bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG540800.10405523331827021RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG538960.1037011992404122No Hit
CCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA532570.10247170045915527RNA PCR Primer, Index 7 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG658250.044.9343871
CAACGGA1497400.044.706881
TCCCTGA879550.044.5745051
CCCTTTA541150.044.56051310
ACCCGTA1388700.044.491582
CCTTTAA542500.044.46206711
CCCGTAG1393100.044.457563
ATCCGTC335650.044.4393845
AACCCGT775600.044.417641
TATAGTT165500.044.40848516
CAACGCT156150.044.4028137
GCGAAGT241000.044.3954411
TTAACCT543750.044.38045514
TCAACGC156200.044.376296
GACCCTT543400.044.363978
GACCCTA339900.044.318158
CCTAACT340150.044.31164611
ATAGTTT151700.044.31082517
TGAGGTA219950.044.272661
ATTGCAC553800.044.2657932
Produced by FastQC (version 0.11.5)