FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005110118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29300191
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC26767689.13566740913054RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG14889755.081792811521263RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA13952614.761951893078103RNA PCR Primer, Index 7 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC13204794.506724887902608No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC12053624.113836664068163RNA PCR Primer, Index 31 (100% over 51bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA11496263.923612648122328RNA PCR Primer, Index 7 (100% over 35bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG9044043.0866829502920305RNA PCR Primer, Index 31 (100% over 37bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC6539602.231930843044675RNA PCR Primer, Index 1 (100% over 33bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC5323861.8170052202048788RNA PCR Primer, Index 2 (100% over 34bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC3162941.0794946695057381RNA PCR Primer, Index 30 (100% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC2853490.973881023505956RNA PCR Primer, Index 30 (100% over 36bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC2286150.7802508864191363RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA1667190.5690031167373619No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1591460.5431568688408891RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG1510770.515617799215029No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1352810.46170688784929764RNA PCR Primer, Index 2 (100% over 34bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1342810.45829394081424246RNA PCR Primer, Index 2 (100% over 34bp)
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1285810.438840142714428RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTCT1214680.4145638504540807No Hit
TATTGCACTCGTCCCGGCCTCCATGGAATTCTCGGGTGCCAAGGAACTCCA1182600.4036151163656237RNA PCR Primer, Index 1 (100% over 28bp)
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1167100.3983250484612882RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG985770.33643807987463287Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC940270.3209091708651319RNA PCR Primer, Index 2 (100% over 34bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA884870.3020014442909263RNA PCR Primer, Index 1 (100% over 32bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA876580.29917211119886555RNA PCR Primer, Index 1 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA874480.298455392321504RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCCGCCCCTCCTCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC859950.2934963802795688RNA PCR Primer, Index 1 (100% over 31bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA820840.2801483444254681RNA PCR Primer, Index 31 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC801280.2734726200249002No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG778160.26558188647985265No Hit
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG766970.2617627987476259RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA754280.2574317689601409No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC700850.2391963929518412No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC666510.22747633283346172RNA PCR Primer, Index 1 (100% over 33bp)
AAGAGCCCAGCGCCGAATCCCCGCCCTGGAATTCTCGGGTGCCAAGGAACT652000.2225241466855967RNA PCR Primer, Index 1 (100% over 25bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC609000.20784847443485946RNA PCR Primer, Index 2 (100% over 34bp)
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGC595350.20318980173200918No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG590470.20152428357890226RNA PCR Primer, Index 1 (100% over 29bp)
CGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC569670.19442535374598752RNA PCR Primer, Index 30 (100% over 36bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA478850.1634289687736165RNA PCR Primer, Index 1 (100% over 28bp)
CCCGGTCAGCCCCTCTCCGGCCCCGTGGAATTCTCGGGTGCCAAGGAACTC439100.14986250430927225RNA PCR Primer, Index 1 (100% over 26bp)
CCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC435680.1486952764232834RNA PCR Primer, Index 1 (100% over 33bp)
GCGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA428680.14630621349874476RNA PCR Primer, Index 7 (100% over 35bp)
AAGAGCCCAGCGCCGAATCCCCGCCTGGAATTCTCGGGTGCCAAGGAACTC420400.1434802933537191RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCACAACCGCGCTTGACTAGCTGGAATTCTCGGGTGCCAAGGAACTCC418850.14295128656328554RNA PCR Primer, Index 1 (100% over 27bp)
ACCTCACCCGGCCCGGACACGTGGAATTCTCGGGTGCCAAGGAACTCCAGT416940.14229941367959RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC405180.13828578796636512RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA401840.1371458636566567RNA PCR Primer, Index 7 (100% over 35bp)
TATTGCACTCGTCCCGGCCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT400340.13663392160139842RNA PCR Primer, Index 1 (100% over 30bp)
CCCCGGCCCCGCGTCCTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC397850.13578409778966968RNA PCR Primer, Index 1 (100% over 31bp)
TCCCGGCCCATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC393820.13440868013454246RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC386220.13181484038790053No Hit
CCCCCCACAACCGCGCTTGACTAGCTGGAATTCTCGGGTGCCAAGGAACTC370580.12647699122507428RNA PCR Primer, Index 1 (100% over 26bp)
CGGCGACCGGCCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA361190.12327223395915746RNA PCR Primer, Index 1 (100% over 28bp)
GAAGAGCCCAGCGCCGAATCCCCGCCCTGGAATTCTCGGGTGCCAAGGAAC350360.11957601232019273RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCGG345740.11799923078999724No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT341810.11665794260522055RNA PCR Primer, Index 1 (100% over 30bp)
CTGGACGAGGCGCCGCCGCCCCCCCTGGAATTCTCGGGTGCCAAGGAACTC339640.1159173330986136RNA PCR Primer, Index 1 (100% over 26bp)
TCCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC336640.11489344898809703RNA PCR Primer, Index 1 (100% over 33bp)
CGCGCGCCGGCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC332560.11350096659779453RNA PCR Primer, Index 2 (100% over 34bp)
TCGTTTCCCGGCCCATGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAG314930.10748394097499228RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCGGCCCCGCGTCCTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT314800.10743957266353657RNA PCR Primer, Index 1 (100% over 30bp)
CCCGCCGCCCCTCCTCCTCCTCCCCTGGAATTCTCGGGTGCCAAGGAACTC311330.10625528004237243RNA PCR Primer, Index 1 (100% over 26bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC310870.10609828447875988RNA PCR Primer, Index 2 (100% over 34bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT307010.1047808869232286No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT305500.10426553192093525RNA PCR Primer, Index 31 (100% over 41bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG303740.10366485324276556RNA PCR Primer, Index 1 (100% over 29bp)
ACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA303070.10343618579141685RNA PCR Primer, Index 1 (100% over 32bp)
ACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAA294740.10059320091121592RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)