FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005110119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46594602
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT31110686.67688501771085No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT21576954.630783196731673No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG19868284.264073336220362No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACG15654813.3597904753001213No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC15604403.3489716255114703No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC14683583.1513478750177972No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA14481923.1080681835204857No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG9361832.0092091354273185RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA9205941.9757524702110345RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC6926601.4865670491186942RNA PCR Primer, Index 4 (100% over 51bp)
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA6135351.3167512408411601RNA PCR Primer, Index 1 (100% over 28bp)
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT6053471.2991783898057547No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT5164131.1083107867301882No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC4423080.9492687586429003No Hit
GACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGATTAAAGCC3723250.7990732488711889No Hit
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAAT3264110.7005339373861376No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3014980.6470663704778506RNA PCR Primer, Index 1 (100% over 29bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC2858390.6134594732668819No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA2717510.5832242112509084No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG2697560.5789425994023943RNA PCR Primer, Index 1 (100% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA2656440.5701175428003441RNA PCR Primer, Index 2 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAGG2553550.548035585753045Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG2528910.5427474195401433RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT2477220.5316538598183541No Hit
GTTCTTGTTGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGTT2305070.49470751998267953No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC2269900.4871594353354494No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC2213120.4749734743951671No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC2108170.45244940604922435RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2039520.43771593971336RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1901370.40806658247665684Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1867930.4008897854734332RNA PCR Primer, Index 1 (100% over 32bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1686930.36204408399067345RNA PCR Primer, Index 1 (100% over 33bp)
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAAC1686200.36188741348193076No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC1635220.3509462319261789No Hit
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1628040.34940528089498435RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1615150.3466388660214331RNA PCR Primer, Index 1 (100% over 30bp)
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT1587260.3406531941189239No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1583320.33980760260598425RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC1573580.33771723170851425RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1565440.33597024822746635RNA PCR Primer, Index 1 (100% over 28bp)
ACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGT1564120.33568695360891804No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTTT1558550.3344915361654983No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG1517830.3257523264175537No Hit
GTTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA1503260.3226253547567592No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC1491590.32012077278822987No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1475710.3167126526802397RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC1426070.30605905808574135RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1421770.3051362044041067RNA PCR Primer, Index 1 (100% over 29bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACAT1384410.2971181082306487No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAAG1380780.2963390480296409No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTCT1371660.29438173975603443No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA1359710.2918170649896312No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCCA1356830.29119896764007125No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1261060.2706450845958508No Hit
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG1259950.270406859575708No Hit
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1253460.26901399436784545RNA PCR Primer, Index 1 (100% over 28bp)
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATGA1244110.26700732415312833No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1196140.25671214017452065RNA PCR Primer, Index 1 (100% over 30bp)
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGGA1176510.25249920580929097No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA1168920.2508702617526382RNA PCR Primer, Index 1 (100% over 22bp)
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGATT1156290.24815964733425558No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG1121870.2407725255384733RNA PCR Primer, Index 1 (100% over 29bp)
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCCA1092590.23448853581794732RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1048640.22505611272310042RNA PCR Primer, Index 1 (100% over 29bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG1039200.22303012696620952No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCAC1036770.22250860732751832No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1013230.2174565199634069RNA PCR Primer, Index 3 (100% over 34bp)
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATGA996220.21380588249256854No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG995860.21372862032387357No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC972400.20869370233058326RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA970520.2082902221162872No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG941010.20195687045465052No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT928510.1992741562638522No Hit
TAATACTGCCGGGTAATGATGGATGGAATTCTCGGGTGCCAAGGAACTCCA913000.19594544449590962RNA PCR Primer, Index 1 (100% over 28bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC901790.1935395864096017No Hit
ATCGATGATGAGCACTGGTGGAGTATGAGTCACATACGATGAATACGTGTC864000.1854292048679802No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA842460.18080635177439652RNA PCR Primer, Index 1 (100% over 28bp)
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC834910.17918599240315433No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCAA831230.1783962013453833No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC787210.16894875505106793No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC785460.16857317506435615RNA PCR Primer, Index 1 (100% over 24bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC752510.1615015404574118No Hit
TTTGTTCGTTCGGCTCGCGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA750050.16097358230466266RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT740440.15891111163477692RNA PCR Primer, Index 1 (100% over 30bp)
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCC738000.15838744582473308No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC736220.15800542732396342RNA PCR Primer, Index 1 (100% over 27bp)
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAAT732660.15724139032242404No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGATA720110.1545479452748625No Hit
GCTTTGCACGTATGAGGCCCCGGGTTGGAATTCTCGGGTGCCAAGGAACTC718820.15427108917037213RNA PCR Primer, Index 1 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTC717530.15399423306588175No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCCA716630.15380107764414427RNA PCR Primer, Index 1 (100% over 28bp)
GGTCAATGATGTAATGGCATGTATTAGCTGAATCTAAAGTTGATGTGAGTT710570.15250049780444525No Hit
GATCGATGATGACTTCCATATATACATTCCTTGGAAAGCTGAACAAAATGA709980.15237387369463956No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC706170.15155618240928423RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC703250.15092950037431374RNA PCR Primer, Index 1 (100% over 31bp)
CCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAGGA694850.14912671643809727No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG679090.14574435038633876RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG671720.14416262209944405No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCTGAGGACACAGTGCA668760.143527355379063No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG665550.14283843437486599No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA649570.13940885255334942No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC646660.13878431668973157No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTTGGAATTCTCGGGTGCCA639130.13716824966119465No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC634460.1361659876395124RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG622530.13360560521581447No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT590840.12680438819930256RNA PCR Primer, Index 1 (100% over 30bp)
ATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAACT584040.12534499167950827No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA571590.12267300834547315No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGGA568050.12191326368663907No Hit
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG556040.11933571189212003RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG553830.11886140802318688No Hit
CCCCCCACAACCGCGCTTGACTAGCTTGCTGTTTTGGAATTCTCGGGTGCC545100.11698780043233334No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG544370.11683112992359071No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA543300.11660148958885838RNA PCR Primer, Index 1 (100% over 23bp)
GTCAATGATGTAATGGCATGTATTAGCTGAATCTAAAGTTGATGTGAGTTC536930.11523437843722756No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGGG507110.10883449546365907No Hit
TCCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAGG503410.10804041206318278No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC497720.10681924056353137No Hit
CTTCTATGATGATTTTATCAAAATGACTTTCGTTCTTCTGAGTTTGCTGAA495220.1062826977253717No Hit
TTCCCTTTGTCATCCTATGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG494260.1060766652755184RNA PCR Primer, Index 1 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGGG486160.10433826647988109No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT481030.10323728057597746RNA PCR Primer, Index 1 (100% over 30bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC476770.10232301157975339No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTAA472280.10135938064241862No Hit
TGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGTG472200.10134221127159751No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG471100.10110613242280725RNA PCR Primer, Index 1 (100% over 29bp)
GTTCTTGTGTTGAAATACAACGATGGTTTTTCATATCATTGGTCGTGGTTG468190.10048159655918941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGA1005550.045.056131
GTCTACG112150.044.926721
TCCTGTA454450.044.8744621
GTAAACA692750.044.8111422
AACATCC693050.044.7982525
TAGTAGG209200.044.765496
GGTAGTA242550.044.7526974
AGTAGGT213000.044.7428557
ACCCGTA626500.044.7361642
AAACATC695250.044.7245754
GTTCTTG411000.044.7235871
TATCTGT365500.044.7189641
ACATCCC527750.044.714926
TCCCTGA872100.044.7042771
TTCTTGT401800.044.7020952
CCCGTAG628550.044.6870733
GTAGGTT209850.044.6856168
CCCTTTA770700.044.6851310
CCTTTAA770450.044.68268611
CTTTAAC771200.044.66548512
Produced by FastQC (version 0.11.5)