FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005110120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72876782
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA843545111.57494989282046RNA PCR Primer, Index 3 (100% over 36bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG24377863.345079095287166RNA PCR Primer, Index 3 (100% over 37bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT23047393.162514777340196RNA PCR Primer, Index 3 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC22592453.100088859576703RNA PCR Primer, Index 3 (100% over 51bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA22584013.0989307403831305RNA PCR Primer, Index 1 (100% over 32bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG20712912.8421823016279726RNA PCR Primer, Index 1 (100% over 29bp)
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT13060551.792141425783592No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT13042081.7896070109132973No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACG12082041.657872324823563No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA11761731.613920054812519No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT10383761.4248378859538555RNA PCR Primer, Index 1 (97% over 35bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC9057731.242882815544737No Hit
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG8172301.1213859580133492RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG7212960.9897473244633661RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA6970160.9564308149610669RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA5289660.7258361106010416RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT5092050.6987204786292567RNA PCR Primer, Index 1 (97% over 35bp)
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT4975390.682712636790137No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG4829890.662747430313265No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4749950.6517782302736693RNA PCR Primer, Index 1 (100% over 30bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA4624990.6346314797489274RNA PCR Primer, Index 3 (100% over 36bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG4464100.6125544895766666RNA PCR Primer, Index 1 (100% over 29bp)
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG4289600.588609963595813No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG3657380.5018580540507401RNA PCR Primer, Index 3 (100% over 38bp)
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAAT3459290.47467655748026855No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT3408000.4676386506747787No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3367240.46204564850297586RNA PCR Primer, Index 1 (100% over 33bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC3237420.4442320189165323No Hit
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC3224460.44245367475199443RNA PCR Primer, Index 1 (100% over 31bp)
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT3157890.43331907822164817RNA PCR Primer, Index 1 (97% over 35bp)
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3136050.43032223898140837RNA PCR Primer, Index 1 (100% over 29bp)
GACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGATTAAAGCC3085720.4234160613732917No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA2920310.40071884623006543RNA PCR Primer, Index 3 (100% over 40bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA2880830.39530148298809353RNA PCR Primer, Index 3 (100% over 40bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2842670.3900652473925097RNA PCR Primer, Index 1 (100% over 33bp)
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG2391950.32821838922580304RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT2371550.32541914378162307No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC2363390.3242994456039511No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC2268290.31125002198917073No Hit
TAATGTGAATTGCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2210780.30335861975903383RNA PCR Primer, Index 1 (100% over 32bp)
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCCA2186340.300005013942575RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC2182760.2995137738107042RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC2103410.28862553234032756RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2050010.28129809573644454RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1868730.256423232299143RNA PCR Primer, Index 1 (100% over 29bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC1851980.2541248322408089RNA PCR Primer, Index 3 (100% over 39bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG1807290.2479925636672596RNA PCR Primer, Index 1 (100% over 29bp)
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT1725900.23682439765246496No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1670490.22922115304158192RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC1634730.22431424043943102RNA PCR Primer, Index 1 (100% over 27bp)
GTTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA1622690.2226621367557091No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG1603920.22008655651123565No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1602850.2199397333433301RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1568540.21523178671637835RNA PCR Primer, Index 1 (100% over 30bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1565400.2148009224666369RNA PCR Primer, Index 3 (100% over 34bp)
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAAG1543720.211826038092626No Hit
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC1538050.21104801252064065RNA PCR Primer, Index 3 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC1527400.209586641737282No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC1514760.20785220730520182No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC1483570.20357238056971286RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1482840.2034722114925437No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC1471800.20195732572275213No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1449130.19884659561395013RNA PCR Primer, Index 1 (100% over 33bp)
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG1424350.19544633570675501RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1395110.19143408390343034Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1351710.18547882643885127RNA PCR Primer, Index 1 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA1345710.1846555189552689RNA PCR Primer, Index 1 (100% over 28bp)
ATCGATGATGAGCACTGGTGGAGTATGAGTCACATACGATGAATACGTGTC1322530.1814748077103624No Hit
CACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG1311690.17998736552335695RNA PCR Primer, Index 3 (100% over 38bp)
GTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT1285210.17635383516248013RNA PCR Primer, Index 1 (97% over 35bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1250190.17154846381663777RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGAGACCCTTTAACCTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA1249110.17140026846959297RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTCGTTCGGCTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1241650.17037662283167224RNA PCR Primer, Index 1 (100% over 32bp)
GCACGGGGTCGGCGGCGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1238460.16993889768623427RNA PCR Primer, Index 1 (100% over 31bp)
ACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG1229830.168754707089015RNA PCR Primer, Index 3 (100% over 37bp)
GACCTATGATGATGACTGGTGGCGTATGAGTCATTGACGGTGAATACAGGT1229530.16871354171483588No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG1160740.15927432141556416RNA PCR Primer, Index 1 (100% over 29bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT1159730.15913573132249445RNA PCR Primer, Index 3 (100% over 41bp)
GCGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1159120.15905202839499694RNA PCR Primer, Index 1 (100% over 32bp)
ATCCCACCGCTGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1127190.15467066040319946RNA PCR Primer, Index 1 (100% over 33bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA1120690.1537787439626519No Hit
ACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGT1111040.15245459109322362No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1105800.15173556922422837RNA PCR Primer, Index 1 (100% over 28bp)
TCCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1101230.15110848335756646RNA PCR Primer, Index 1 (100% over 33bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1075560.1475860995069733RNA PCR Primer, Index 1 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1064780.14610689039480365No Hit
TATTGCACTCGTCCCGGCCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG1058290.1452163461333954RNA PCR Primer, Index 1 (100% over 29bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC1034100.1418970447954192No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCAC1024350.14055917013459787No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTCT1021850.1402161253497719No Hit
CCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA990650.13593492643514363RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC971640.13332641389132688RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC963490.13220808789279417RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTCGTCCCGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC962810.1321147797113215RNA PCR Primer, Index 1 (100% over 31bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC958420.13151239306916707No Hit
TCGTTTCCCGGGCGGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAG926740.12716532955585222RNA PCR Primer, Index 1 (100% over 29bp)
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATGA924900.1269128485942203No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAAC883160.12118537286676571No Hit
TCGGATCCGTCTGAGCTTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT882550.12110166993926817RNA PCR Primer, Index 1 (100% over 30bp)
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG882260.12106187674422837No Hit
CCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA876100.12021661439441714RNA PCR Primer, Index 1 (100% over 32bp)
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCC871590.11959776160259106No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGGTGCCAAGGAACTCC862860.11839984921397874RNA PCR Primer, Index 1 (100% over 27bp)
ACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC843330.11571998335491818RNA PCR Primer, Index 3 (100% over 39bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC816350.11201784403707617RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG800870.1098937107294337RNA PCR Primer, Index 3 (100% over 37bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT787220.10802068620428384RNA PCR Primer, Index 1 (97% over 35bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT770360.1057071921754174RNA PCR Primer, Index 1 (100% over 30bp)
TCGGATCCGTCTGAGCTTGGCATGGAATTCTCGGGTGCCAAGGAACTCCAG770120.10567425987607411RNA PCR Primer, Index 1 (100% over 29bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC736870.10111176423788855RNA PCR Primer, Index 3 (100% over 42bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC731350.10035432135299278RNA PCR Primer, Index 3 (100% over 44bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG730560.10024591920098777RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)