FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005110121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56668982
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACAT44516477.855526679480496No Hit
GACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACG38376206.7719938925318965No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT35292406.2278161269951875No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC20132933.5527248398427207RNA PCR Primer, Index 6 (100% over 36bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA15899142.8056159540681356No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC11642462.054467821567714No Hit
GACCAATGATGAGATTGGAGGGTGTCTGAATCAAAAATTTTGATTAAAGCC9615391.6967642016226796No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG8803581.5535094666073233RNA PCR Primer, Index 6 (100% over 34bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7884261.391283153807139RNA PCR Primer, Index 1 (100% over 32bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT7369031.3003639274832923No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC6513541.149401272110376RNA PCR Primer, Index 6 (100% over 51bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG6486521.144633231632783No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT5663950.9994797506685403No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC5315840.938051084100999No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGG4922550.8686498021086739No Hit
TTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAAT4080200.7200058755246388No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG4002500.7062946710424408RNA PCR Primer, Index 1 (100% over 29bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG3530280.622965134612794RNA PCR Primer, Index 1 (100% over 29bp)
ACCAATGATGACAAATACCGGCGTATGAGTCTTGGATGATGAATAATACGT3471120.6125255611614834No Hit
GTTGGAGGATGAAAGTACGGAGTGATCCATCGGCTAAGTGTCTTGTCACAA3417190.6030088911778934No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT3379040.596276813301499No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC3201500.564947505144878No Hit
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC3161260.5578466188081516No Hit
GATCGATGATGACTTCCATATATACATTCCTTGGAAAGCTGAACAAAATGA2979480.5257691059281777No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTCT2816790.49706027893707355No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC2795110.4932345528987974RNA PCR Primer, Index 5 (97% over 35bp)
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGGA2777720.4901658547527817No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAAC2775000.4896858743642157No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2671590.47143779642979994RNA PCR Primer, Index 6 (100% over 34bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2591260.4572624932630694RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCAG2431770.4291183490820428RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA2421400.4272884238506349RNA PCR Primer, Index 6 (100% over 37bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2420910.4272019567953418RNA PCR Primer, Index 1 (100% over 30bp)
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAAG2291330.4043358322547598No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2215390.39093520331810444RNA PCR Primer, Index 1 (100% over 29bp)
GACCTATGATGATGACTGGTGGCGTATGAGTCATTGACGGTGAATACAGGT2145550.3786110009881596No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGACCCTGTTGATCTC1986230.35049685558141846No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTGG1828860.3227268137620683No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATGGATGAGAAATGCGG1771740.31264722560218217No Hit
GACCAATGATGACCACTGGTGGCGTTTGAGTCATGGACGATGAATACTACG1754260.30956264575213294No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGATT1659810.2928956796859347No Hit
GATCGATGATGACTTTCATACATGCATTCCTTGGAAAGCTGAACAAAATGA1651220.2913798592676325No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG1596430.28171143077883415No Hit
ATAGGCGATAGAAATTGAAACCTTGGAATTCTCGGGTGCCAAGGAACTCCA1554580.27432643840328735RNA PCR Primer, Index 1 (100% over 28bp)
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTAATAATGAAAACAAC1540680.27187359744701256No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCCCTGAGTGCAATCAC1436390.2534702317398255No Hit
CTCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATG1411410.24906217655365684No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA1404900.2479133999619051RNA PCR Primer, Index 1 (100% over 28bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC1382240.2439147398130427No Hit
GTCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCTTGATGAAAGCACA1373530.24237774378936255No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC1371590.24203540483575303No Hit
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1348350.23793439592756405RNA PCR Primer, Index 1 (100% over 31bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC1311430.2314193680062931No Hit
GGGCGGTGATGACCCCAACATGCCATCTGAGTGTCGGTGCTGAAATCCAGA1303150.22995825123521715No Hit
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1285030.22676073482315248RNA PCR Primer, Index 1 (100% over 30bp)
ATCGATGATGAGCACTGGTGGAGTATGAGTCACATACGATGAATACGTGTC1248140.22025100080322602No Hit
CACCAGTGATGAGTTGAATACCGCCCCAGTCTGATCAATGTGTGACTGAAA1216640.2146924043915241No Hit
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1202100.21212662687323378RNA PCR Primer, Index 5 (97% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1198310.21145783067004803RNA PCR Primer, Index 5 (97% over 35bp)
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA1168730.20623804394439274RNA PCR Primer, Index 1 (100% over 28bp)
GATCGATGATGACTTTTATACATGCATTCCTTGGAAAGCTGAACAAAATGA1131370.19964537213673614No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTAA1074570.18962225225785775No Hit
GCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAAT1055920.1863312102553739No Hit
GATCGATGATGACTTCCATATGTACATTCCTTGGAAAGCTGAACAAAATGA1037330.18305075605557905No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA1033470.18236960741592287No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1009730.17818036681865929RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAGGA1003530.17708629387413383No Hit
TAATGTGAATTGCAGGACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA987850.17431934810475333RNA PCR Primer, Index 1 (100% over 32bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTA984780.1737776055338351No Hit
TGGCAATGATGACCCACTTGCCCTCACTGAGAACAAAGTTCGGTAATGAGA960500.16949307471237088No Hit
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG942000.16622850221660943RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC940740.16600615836014135RNA PCR Primer, Index 6 (100% over 36bp)
TCCCTGAGACCCTTTAACCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT939580.1658014608414882RNA PCR Primer, Index 1 (100% over 30bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC921600.16262864930236437No Hit
CCACAATGATGACAGTTTATTTGCTACTCTTGAGTGCTAGAATGATGAGGA921220.16256159321866764No Hit
TTTGTTCGTTCGGCTCGCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG919190.16220337256102466RNA PCR Primer, Index 1 (100% over 29bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACACAT907840.16020051321903048No Hit
TCCCAATGATGAGTTGCCATGCTAATACTGAGCCACCAGGTAGGGCAGTGT900740.1589476232341707No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC896350.1581729490040954No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA885360.15623361647823494RNA PCR Primer, Index 6 (100% over 40bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC883650.1559318641015997RNA PCR Primer, Index 5 (97% over 35bp)
GATCGATGATGACTTCCTTATATACATTCCTTGGAAAGCTGAACAAAATGA866250.15286140132180248No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCTGAGGACACAGTGCA865390.1527096428165941No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCC863610.1523955380034884No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATTAAT837590.1478039609040445No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAAC834840.147318686614134RNA PCR Primer, Index 1 (100% over 24bp)
GCGTTCGCGCTTTCCCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA833710.14711928299682533RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGAGACCCTAACTTGTGAATGGAATTCTCGGGTGCCAAGGAACTCCA828370.14617696855750822RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGG818760.14448115549349377No Hit
CACCCGTAGAACCGACCTTGCGATGGAATTCTCGGGTGCCAAGGAACTCCA814100.1436588361513182RNA PCR Primer, Index 1 (100% over 28bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCT813020.143468255702917No Hit
AAGCTATGATGAATTTGATTGCATTGATCGTCTGACATGATAATGTATTTT808750.14271475707821962No Hit
TCCTGGTGATGACAGATGGCATTGTCAGCCAATCCCCAAGTGGGAGTGAGG783900.13832964213121032No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC767940.13551328661594803No Hit
TGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGTG767750.13547975857409966No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC750040.13235459214707618RNA PCR Primer, Index 1 (100% over 27bp)
GGTCAATGATGTAATGGCATGTATTAGCTGAATCTAAAGTTGATGTGAGTT719080.12689128595957486No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA698890.12332849035474114RNA PCR Primer, Index 1 (100% over 22bp)
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAGCGCCCGAATCGGAC696520.12291027214852739No Hit
TGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAA694830.12261204903945513No Hit
TGCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGATTGCTCTGTCCAAA684930.12086506159577738No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTC681220.1202103824628436No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG651940.11504353475063307No Hit
GGTCAGTGATGAGCAACATTCACCATCTTTCGTTTGAGTCTCACGGCCATG639790.11289950470611948No Hit
CCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA632960.11169425983335998RNA PCR Primer, Index 1 (100% over 28bp)
ATCGATGATGACTACCGGTGGCGTATGAGTCATATGTGATGAATACGTGTT630510.11126192455689428No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCG629750.11112781238950084No Hit
ATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAACT619760.10936494324178965No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG610790.10778206673979072Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGAGCTCCGTGAGGATA606380.10700386324215248No Hit
TCGCTGTGATGAGTGATTGTTAAACATTCGTAGTTTCCACCAAAAGCTTGG597260.10539451723343114No Hit
GATCGATGATGAGTCCCCCATAAAAACATTCCTTGGAAAAGCTGAACAAAA596890.10532922578351592No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATGA589520.10402869068655582No Hit
GTCTGTGATGAATTGCTTTGACTTCTGACACCTCGTATGAAAACTGCACGT584320.10311108112017965No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTCCTCCCGG580600.10245463735346436No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGA576370.10170819726389296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)