FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005110159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4389558
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGACGTTGTAGTAGTACCAGTTGCTATTCGGGCCT247670.5642253730330024No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA235230.5358853898274041No Hit
ATTTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGACT153900.35060477615286095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC124300.2831720186861638No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTGATCACTACTGGGGCTGGATCCG121810.2774994657776478No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT119320.2718269128691317No Hit
ATATTACTGTGCGAGACGTTGTAGTAGTACCAGTTGCTATTCGGGCCTAG112710.2567684491240348No Hit
ACTACAACCCGTCCCTCAAGAGTCGACTGACCATATCCGTAGACACGTCC102650.23385042412015059No Hit
TTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGACTGA96930.220819499366451No Hit
ATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACT95130.2167188587096924No Hit
GGGTATTAGCAACTGGTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCC87010.19822041308031468No Hit
GCTGTATATTACTGTGCGAGACGTTGTAGTAGTACCAGTTGCTATTCGGG85850.1955777779904036No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC84870.19334520696616836No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTG83920.19118097995287908No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT83770.1908392598981492No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA81440.185531208381345No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG72970.16623541595759755No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA72380.16489131707566002No Hit
ATAATAAATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA71000.16174749257214507No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG70100.15969717224376576No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC69320.15792022795917038No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT67470.1537056806175018No Hit
ATTTTACATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGT66670.1518831736589424No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC65880.15008344803736504No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA64850.14773697032821984No Hit
ATAGGAGACAGAGTTACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAA64030.14586890069569647No Hit
TCCGTAGACACGTCCAAGAATCAGTTCTCCCTGAAGCTGACCTCTGTGAC61370.13980906505848653No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG56560.12885124197014824No Hit
TTGTAGTAGTACCAGTTGCTATTCGGGCCTAGCGGCTTTTGATATCTGGG56250.1281450205237065No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT56170.12796276982785054No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT56000.1275754870991567No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGACTGACCATATCCGTAGACAC55950.1274615804142467No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55030.12536569741190343No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTGATCACTACTGGG53950.12290531301784827No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA53310.12144730745100075No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC53090.12094611803739692No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG52070.11862242166523372No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA51250.11675435203271035No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG51120.11645819465194444No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50950.11607091192325059No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA50100.11413449827978124No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50090.11411171694279926No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG47970.10928207350261689No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46350.10559149691153413No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC45650.10399680332279469No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC45060.10265270444085714No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC44800.10206038967932535No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG44620.10165032561364948No Hit
GACCATATCCGTAGACACGTCCAAGAATCAGTTCTCCCTGAAGCTGACCT44060.10037457074265792No Hit
TTATTATTGTCAACAGGCTAACAGTTTCCCTTGGACGTTCGGCCAAGGGA44040.10032900806869394No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)