FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4359489
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAA219960.5045545475627992No Hit
GTAGTACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTCCAGGGAGA131110.30074625718748227No Hit
CTATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGA116850.26803600146714446No Hit
ATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA98260.2253933889958204No Hit
GTCTCTGGTGGCTCCATCAGCACTAGTAGTTACTACTGGGGCTGGATCCG85590.19633034972676844No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC84580.19401356443381323No Hit
GTGTACTACTGTGCGAGACTAGAGGACCTCTGGGGGTCCTCTGACTACTG78510.1800899142078349No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT76420.17529577434419494No Hit
ACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTC72020.16520284831547918No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA63430.14549870409123639No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA62310.14292959564756327No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT61530.14114039512429094No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC60870.1396264562199836No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA58960.13524520878479107No Hit
ACTACAACCCGTCCCAACCGTCCCTCAAGAGTCGAGTCACCATGTCCGTA58200.1335018851980129No Hit
ATTATAGTGGGAGTACCTACTACAACCCGTCCCAACCGTCCCTCAAGAGT57730.13242377719040005No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA57600.132125577103188No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56360.12928120704054993No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG55170.12655152931914726No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC55060.12629920616842935No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT54950.12604688301771147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53580.12290431286786135No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACAATGCCAGTG50920.11680268031413774No Hit
GTACTTGATGAAGCTTTACAGTGATGGCGGCCACATCAAGGGGGACGGGA50680.11625215707620778No Hit
CTATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG50630.11613746473497237No Hit
CATCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG49910.11448589502118253No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49290.11306370998986348No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAG48910.1121920481964744No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATTGTGGTTTTCGGCGGAGG48830.11200854045049775No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA48660.11161858649029736No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATTGTGGTTTTCGGCGGAGGGA48450.11113687865710867No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT47750.10953118587981298No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC46680.10707676977737529No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC46280.10615923104749204No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA44660.10244319919146486No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC44580.10225969144548822No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44020.10097513722365167No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT43760.10037873704922756No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)