FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3349486
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT103750.3097490182075697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC100390.2997176283167029No Hit
ATATTACTGTCAACAGTTTGATAATCTCCCGCTCACCTTCGGCCAAGGGA80000.23884261644921043No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA65610.1958808008154087No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA63860.1906561185805822No Hit
ATCTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGT61040.18223691635074754No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT54190.1617860173172839No Hit
ATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC51800.15465059415086374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51080.15250101060282087No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51020.15232187864048394No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG48780.14563428537990605No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT47610.14214121211433636No Hit
ACATATTACTGTCAACAGTTTGATAATCTCCCGCTCACCTTCGGCCAAGG47520.141872514170831No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA46640.13924524538988967No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG42630.127273259240373No Hit
ATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC40280.12025725738217745No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT40200.12001841476572825No Hit
CTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG38990.11640592019193391No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38950.11628649888370932No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA38740.11565953701553014No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38200.11404734935449798No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAATAGACACGTCC38200.11404734935449798No Hit
ATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA38020.11350995346748727No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA36070.10768816469153775No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG35950.10732990076686394No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA35720.10664322824457245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT35360.105568436470551No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC35220.10515046189176488No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG34690.10356812955778887No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC33590.10028404358161222No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)