FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074300
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC61020.14976805831676607No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTG60670.1489090150455293No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGAAG59480.14598826792332426No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGC54260.13317625113516432No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT53940.13239084014431926No Hit
ATGTATTACTGTGCGAGACCACGGGGGCTACGGGCCAATCCCTACTACTT52260.12826743244238273No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT48920.1200697052254375No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC47250.11597084161696489No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT46390.11386004957906881No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG45200.11093930245686374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44440.10907395135360674No Hit
CCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCCCTCAGGGATCCCA44180.10843580492354515No Hit
GTACTTGTCTTCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCG43580.10696315931571067No Hit
AGCCAGGACAGGCCCCTGTACTTGTCTTCTATGGTAAAAACAACCGGCCC43570.10693861522224676No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG41690.10232432565103208No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGA41290.10134256191247576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)