FastQCFastQC Report
Sun 7 Aug 2022
EGAF00005110202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074300
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCAAGGAAG62290.15288515818668238No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC60800.14922808826056008No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGC59050.14493287190437623No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTACTTG57530.1412021696978622No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG54870.1346734408364627No Hit
ATGTATTACTGTGCGAGACCACGGGGGCTACGGGCCAATCCCTACTACTT54380.1334707802567312No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49350.12112510124438554No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT49050.1203887784404683No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC45830.11248558034508994No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC44420.10902486316667895No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT43790.10747858527845275No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT42780.10499963183859805No Hit
GTACAGGGGCCTGTCCTGGCTTCTGCTGGTACCAGCTTGCATAATAGCTT42470.1042387649412169No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA40870.10031170998699164No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG40860.10028716589352772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)