FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049400
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT95520.4660876354054845No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC89550.43695715819264175No Hit
GTACTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT72830.35537230408900167No Hit
TTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGGGA52390.25563579584268564No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT48120.23480042939396897No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45460.22182102078657168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45090.22001561432614422No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43120.21040304479359812No Hit
TTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTACTACACGT41860.20425490387430467No Hit
CTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG37860.18473699619400802No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA37690.1839074851175954No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37270.18185810481116424No Hit
CTTTAATACTACCGGCTACTACACGTTTGACTCGTGGGGCCAGGGAACCC36500.17810090758270714No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG35850.17492924758465894No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACA35660.17400214696984484No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA34900.17029374451058849No Hit
GTTTATTACTGCATGCAAACTCTACAAACTCCGTACACTTTTGGCCAGGG34070.1662437786669269No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA33080.16141309651605346No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT32650.1593149214404216No Hit
ATCCAGTACTTTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC32610.15911974236361862No Hit
GTACAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAG32390.15804625744120232No Hit
GTATTAAAGTACGCCACCCACGTAGTACAATAATACACGGCTGTGTCCTC30620.149409583292671No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA30130.1470186396018347No Hit
GTATTATTGTACTACGTGGGTGGCGTACTTTAATACTACCGGCTACTACA30000.14638430760222504No Hit
GGTCTAGTCAGAGCCTCCTGCAAAGTACTGGATACAACTATTTGGATTGG29770.14526202791060797No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA28640.13974821899092418No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28360.1383819654533034No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC28190.1375524543768908No Hit
CAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCC28180.13750365960769007No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC28160.13740607006928857No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27810.1356982531472626No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTGACGCCTGG27410.13374646237923293No Hit
CTCTACAAACTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA27070.13208744022640773No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG27060.13203864545720698No Hit
AGTCAGAGCCTCCTGCAAAGTACTGGATACAACTATTTGGATTGGTACCT26740.13047721284278324No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT26590.12974529130477214No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26340.1285254220747536No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT26180.1277447057675417No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25900.12637845222992095No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25840.1260856836147165No Hit
AAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTC25180.12286522884746756No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG25170.12281643407826681No Hit
GAGTTTGTAGAGTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCC25090.12242607592466087No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA24980.12188933346345271No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGT24950.1217429491558505No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG24590.11998633746462378No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC24560.11983995315702158No Hit
ATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAGATCCA24550.11979115838782083No Hit
CAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTCCATTTGCAGATC24170.11793695715819263No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC23690.11559480823655705No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23600.11515565531375037No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG22930.11188640577730066No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22840.111447252854494No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT22720.11086171562408509No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG22340.10900751439445691No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC22210.10837318239484726No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT22000.10734849224163169No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21410.10446960085878794No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC21390.10437201132038647No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21260.10373767932077682No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG21210.1034937054747731No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21010.10251781009075828No Hit
CTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT21000.10246901532155751No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG20950.10222504147555382No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA20560.10032204547672491No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20530.10017566116912266No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)