FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3461322
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT110680.31976221801958904No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC90920.26267420367131405No Hit
AATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA66720.19275872051198933No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCACATACA66440.19194978103741864No Hit
TTTAAGAGGTGTCCAGTGTCTGGTGCAGCTGGTGGAGTCTGGGGGAGGCG58180.16808606653758304No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55700.1609211740485283No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT54840.1584365742337754No Hit
GTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT54030.15609642789662445No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG53940.155836411636941No Hit
GTGTATTATTGTGCGAGAGATAAGGGTCCCTACGGTGACTACGGGGGGTA51570.14898931679861047No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAA49590.14326895908557483No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46510.13437062486529713No Hit
ATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT46340.13387948304145064No Hit
ACAGTAGTCCGCTCACTTTCGGCGGAGGGACCGAGGTGGAGATCAAACGA46330.13385059234593025No Hit
GTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGG44470.12847692297913918No Hit
GTATTATTGTGCGAGAGATAAGGGTCCCTACGGTGACTACGGGGGGTACT43870.12674348124791626No Hit
GAGTTACAGTAGTCCGCTCACTTTCGGCGGAGGGACCGAGGTGGAGATCA42780.1235943954361946No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA42060.12151426535872709No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA40520.11706509824858827No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC38360.11082470801618573No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC37920.1095535174132889No Hit
ATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGGTCCTGATC37430.1081378733327902No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG37410.10808009194174942No Hit
AGTCAGAACATTAACAACTATTTAAATTGGTATCAGCAGAAATCAGGGAA36820.10637554090604688No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36760.10620219673292458No Hit
ACTTACTACTGTCAAGAGAGTTACAGTAGTCCGCTCACTTTCGGCGGAGG36380.10510435030315007No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36360.10504656891210931No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT36140.10441097361066089No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35970.1039198317868144No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG35960.10389094109129401No Hit
CTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCA35550.10270642257495836No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT35230.10178192031830612No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCACATA35090.10137745058102078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)