FastQCFastQC Report
Thu 28 Jul 2022
EGAF00005110260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3461322
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT107800.311441697709719No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCACATACA80050.2312700176406587No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC76800.2218805415965345No Hit
AATGAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA63790.1842937467245174No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG58070.16776826888685883No Hit
GTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT57230.16534145046314674No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT53600.15485412798924802No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG53060.1532940304311474No Hit
ACAGTAGTCCGCTCACTTTCGGCGGAGGGACCGAGGTGGAGATCAAACGA50730.14656249837489838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT50680.14641804489729648No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTAACAA48690.14066879648874042No Hit
GAGTTACAGTAGTCCGCTCACTTTCGGCGGAGGGACCGAGGTGGAGATCA47140.1361907386830812No Hit
GTGTATTATTGTGCGAGAGATAAGGGTCCCTACGGTGACTACGGGGGGTA45570.1316548994863812No Hit
ATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT44290.12795689045977232No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42290.12217875135569589No Hit
GTTATATGGTATGATGGAAGTAATGAATACTATGCAGACTCCGTGAAGGG41640.12030085614687105No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40280.11637172155609908No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA40110.11588057973225259No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39470.11403157521894813No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA39450.11397379382790736No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCACATA39170.11316485435333669No Hit
GTATTATTGTGCGAGAGATAAGGGTCCCTACGGTGACTACGGGGGGTACT39110.11299151018021437No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG38120.11013133132369654No Hit
ACTTACTACTGTCAAGAGAGTTACAGTAGTCCGCTCACTTTCGGCGGAGG37970.10969797089089083No Hit
ATTTAAATTGGTATCAGCAGAAATCAGGGAAAGCCCCTAAGGTCCTGATC37790.10917793837152395No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37330.10784896637758637No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG36730.10611552464636345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT35420.10233084353319338No Hit
AGTCAGAACATTAACAACTATTTAAATTGGTATCAGCAGAAATCAGGGAA35120.10146412266758194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)