FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3394323
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACACCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA135660.399667326886687No Hit
GTATATTACTGTGCGAGATTAGAGGGAACGTTTCACTATGATAGTAGTCG125160.36873332325768643No Hit
GTTTATGGTGGGTCCTTCAATGGTTACTACTGGAATTGGATCCGCCAGCC123460.3637249607653721No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC121300.3573613943045491No Hit
ACTCAGGACTCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCTC106410.3134940310630426No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCACAAGATGATTA93360.27504748369557047No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACTCTGCT83460.24588113741679857No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA82700.24364210477317566No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC82110.2419039083787842No Hit
AGCCAGGACAGGCCCCTGTTCTTGTCATCTATGGTAAAAACAACCGGCCC79400.23391998934691838No Hit
ATATACACCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACT78970.23265316824592122No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT78480.2312095814099012No Hit
GACTATTACTGTAGCTCCCGGGACAGCAGTGGTAATCATCTTGTGGTGTT75360.22201776318871244No Hit
GTAATAGTCAGCCTCATCTTCCTCCTTAGCCCCATTGATGGTCAAGGAAG73780.2173629321664438No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA70410.20743459004932646No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC69590.2050187916706807No Hit
ATATTACTGTGCGAGATTAGAGGGAACGTTTCACTATGATAGTAGTCGTT69550.20490094784733215No Hit
CTATTACTGTAGCTCCCGGGACAGCAGTGGTAATCATCTTGTGGTGTTCG66310.1953555981560977No Hit
GGTGTATATTACTGTGCGAGATTAGAGGGAACGTTTCACTATGATAGTAG65720.19361740176170625No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG63940.18837335162269472No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA63530.18716545243337182No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCAC62930.18539779508314325No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA62570.18433720067300607No Hit
GTCTGTGGCCTTGGGACAGACAGTCAGGATCTCATGCCAAGGAGACAGCC61790.1820392461177089No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACTCTGCTGTGT60600.17853339237308882No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC60490.17820932185888025No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT60150.17720764936041739No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT59050.17396694421833162No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG58400.17205198208891728No Hit
CTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCTCATGCCAAGGA57520.1694594179752487No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG57430.16919426937271437No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT57030.16801583113922866No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA56820.16739715106664863No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA55940.16480458695298003No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCACAAGATGATTAC55410.1632431562936114No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA54840.16156388181089426No Hit
GTCAGGATCTCATGCCAAGGAGACAGCCTCAGCAGCTATTTTCCAAGTTG54340.16009083401903706No Hit
GTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCATG52480.15461109623332842No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52450.15452271336581697No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG51520.15178284447296264No Hit
ATCTTGTGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC50960.15013303094608263No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT50730.14945542896182834No Hit
CTTCTGAGCTGACTCAGGACTCTGCTGTGTCTGTGGCCTTGGGACAGACA50350.1483359126400169No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG48980.14429976169032824No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT48770.14368108161774823No Hit
GTTCTTGTCATCTATGGTAAAAACAACCGGCCCTCAGGGATCCCAGACCG48060.14158935375331103No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC46990.1384370314787367No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA46360.13658099126099668No Hit
CAGTAATAGTCAGCCTCATCTTCCTCCTTAGCCCCATTGATGGTCAAGGA45840.1350490215574652No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG45820.13499009964579092No Hit
CCACAAGATGATTACCACTGCTGTCCCGGGAGCTACAGTAATAGTCAGCC45450.13390004427981664No Hit
CAGTAATATACACCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGA44570.13130748016614802No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA44160.1300995809768251No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC43570.12836138458243365No Hit
TCATAAGGGTATCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT43570.12836138458243365No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42940.12650534436469363No Hit
GTCCTGGCTTCTGCTGATACCAACTTGGAAAATAGCTGCTGAGGCTGTCT42210.12435469458858218No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG42110.12406008503021072No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA40770.12011231694803352No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA40470.11922848827291924No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40350.1188749568028735No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT39920.11760813570187634No Hit
CTATGATAGTAGTCGTTACAAACGCGGGGATTTCTTTGACTTCTGGGGCC39620.11672430702676204No Hit
CCTTAGCCCCATTGATGGTCAAGGAAGCTGTGTTTCCTGAGCTGGAGCCA39340.11589940026332203No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG39070.11510395445571917No Hit
GTATCAACGCAGAGTACGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGG38600.11371928953137342No Hit
GTTCTGTGGTTTCTTCTGAGCTGACTCAGGACTCTGCTGTGTCTGTGGCC38130.11233462460702767No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC37190.10956529475833619No Hit
ACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGG36990.10897607564159333No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT36830.10850470034819903No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA35840.10558806572032184No Hit
GGGCTAAGGAGGAAGATGAGGCTGACTATTACTGTAGCTCCCGGGACAGC35540.10470423704520755No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG35540.10470423704520755No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC34500.10164029763814464No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34190.1007270080071932No Hit
GCCCTCAGGGATCCCAGACCGATTCTCTGGCTCCAGCTCAGGAAACACAG34030.10025563271379889No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)