FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085895
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC269160.6587540795835429No Hit
GTATATTACTGTGCGAGAGAACCCTCATGGTTCGGGGAGTCAAACTGGGG239210.5854531259369122No Hit
GTAATATACAGCCGTGTCCGCGGCGGTCAGAGAGGTCAGCTTCAGGGAGA236690.5792855665649753No Hit
GTTTTATACAGCTCCAACAATAAGGACTACTTAGCTTGGTACCAGCAGAA149580.36608870271996713No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC112220.27465218758680776No Hit
ATATACAGCCGTGTCCGCGGCGGTCAGAGAGGTCAGCTTCAGGGAGAACT105660.2585969536662102No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT104240.25512158290900766No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG92890.22734309129333963No Hit
GCTGTATATTACTGTGCGAGAGAACCCTCATGGTTCGGGGAGTCAAACTG90290.22097973638578575No Hit
ATATTACTGTGCGAGAGAACCCTCATGGTTCGGGGAGTCAAACTGGGGCC88740.2171861978832055No Hit
ATATTATGGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA88700.21708830011539698No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT85920.21028440525270473No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC85900.21023545636880045No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT75030.18363173796683468No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT73570.18005846944182366No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA66950.1638563888695133No Hit
TTATTACTGTCAGCAATATTATGGTACTCCTCGGACGTTCGGCCAAGGGA66050.16165368909382155No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC65680.16074813474159272No Hit
CAGTAATATACAGCCGTGTCCGCGGCGGTCAGAGAGGTCAGCTTCAGGGA60170.14726271722596884No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA59390.14535371075370268No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53200.13020403118533394No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA52980.12966559346238707No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG50190.12283722415774269No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG49970.1222987864347958No Hit
TTATGGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC49220.12046320328838601No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT49180.12036530552057749No Hit
GTTTATTACTGTCAGCAATATTATGGTACTCCTCGGACGTTCGGCCAAGG47570.1164249203662845No Hit
CTACAATCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA47040.11512777494282159No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTGCTCATT45990.11255795853784788No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTC45970.11250900965394361No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG45680.11179925083733185No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT45460.11126081311438499No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45250.11074684983339023No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGT44330.10849520117379424No Hit
CCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCT43470.10639039916591102No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT42720.10455481601950123No Hit
ACTACAATCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCC42090.10301292617651701No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG41830.10237659068576162No Hit
GACCTACACCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA41680.10200947405647968No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)