FastQCFastQC Report
Tue 26 Jul 2022
EGAF00005110314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005110314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4085895
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGCCAGCC255350.6249548752476508No Hit
GTAATATACAGCCGTGTCCGCGGCGGTCAGAGAGGTCAGCTTCAGGGAGA220250.5390495839956729No Hit
GTATATTACTGTGCGAGAGAACCCTCATGGTTCGGGGAGTCAAACTGGGG198690.4862826871468797No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT123400.3020146136892896No Hit
ATATACAGCCGTGTCCGCGGCGGTCAGAGAGGTCAGCTTCAGGGAGAACT103290.2527965109235553No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC101470.24834216248826754No Hit
ATATTATGGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA96420.23598256930244169No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC91600.2241858882815148No Hit
GTTTTATACAGCTCCAACAATAAGGACTACTTAGCTTGGTACCAGCAGAA87420.21395557154552428No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT80160.1961871266882776No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT75340.18439044566735074No Hit
GCTGTATATTACTGTGCGAGAGAACCCTCATGGTTCGGGGAGTCAAACTG75200.1840478034800209No Hit
TTATTACTGTCAGCAATATTATGGTACTCCTCGGACGTTCGGCCAAGGGA73630.18020531609353643No Hit
ATATTACTGTGCGAGAGAACCCTCATGGTTCGGGGAGTCAAACTGGGGCC72370.17712153640756798No Hit
TCATAAGGGTATCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT62830.15377291878523555No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA62450.15284288999105458No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA62060.1518883867549215No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT58200.14244125216139913No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56480.1382316481456327No Hit
CCATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT54390.13311648977763746No Hit
CAGTAATATACAGCCGTGTCCGCGGCGGTCAGAGAGGTCAGCTTCAGGGA53540.13103616221170636No Hit
TTATGGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC52650.12885793687796676No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTC50650.12396304848754068No Hit
GTTTATTACTGTCAGCAATATTATGGTACTCCTCGGACGTTCGGCCAAGG50440.12344908520654593No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG49580.12134428319866271No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA48440.11855419681611984No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT47580.11644939480823663No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46830.11461381166182685No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC46200.11307192181884262No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG45350.11099159425291155No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA45050.11025736099434763No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG44760.10954760217773585No Hit
GGGGAGGTGTGTCACAAGATTTGGGCTCTGGGCACCGTGGGCATGTGTGA44740.1094986532938316No Hit
CTACAATCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA44380.10861757338355489No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGAACTGCT44320.10847072673184212No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA43570.10663514358543233No Hit
ACTACAATCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCC42730.10457929046145337No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT41750.10218079515014458No Hit
CCATATCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGACCTCT41730.10213184626624032No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA41310.10110391970425084No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA41030.10041863532959118No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41000.10034521200373481No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT40970.1002717886778784No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)